Restriction map for
pF1KB7861
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AhdI AlwNI AocI ApoI
AscI AseI AsnI AspEI AviII
AvrII BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 111 GGTACC G^GTACC
AflIII 1175 ACGTGT A^C[GA][CT]GT
AgeI 923 ACCGGT A^CCGGT
Alw44I 588 GTGCAC G^TGCAC
ApaLI 588 GTGCAC G^TGCAC
Asp718I 111 GGTACC G^GTACC
AspI 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 287 GGATCC G^GATCC
BfaI 1303 CTAG C^TAG
BlpI 763 GCTGAGC GC^T[ACGT]AGC
BpmI 546 CTCCAG CTGGAG(16/14)
Bpu1102I 763 GCTGAGC GC^T[ACGT]AGC
BsaAI 1174 TACGTG [CT]AC^GT[GA]
BsaI 320 GAGACC GGTCTC(1/5)
BssHII 704 GCGCGC G^CGCGC
BssSI 1564 CACGAG CACGAG(-5/-1)
BstEII 1221 GGTCACC G^GT[ACGT]ACC
BstPI 1221 GGTCACC G^GT[ACGT]ACC
BstXI 1589 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 763 GCTGAGC GC^T[ACGT]AGC
DraIII 964 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 491 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1221 GGTCACC G^GT[ACGT]ACC
FseI 737 GGCCGGCC GGCCGG^CC
KpnI 111 GGTACC GGTAC^C
MaeI 1303 CTAG C^TAG
MaeII 1175 ACGT A^CGT
MseI 146 TTAA T^TAA
NlaIII 1602 CATG CATG^
PflFI 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 61 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 923 ACCGGT A^CCGGT
PshAI 799 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 951 CAGCTG CAG^CTG
SexAI 965 ACCTGGT A^CC[AT]GGT
SgrAI 976 CACCGGCG C[GA]^CCGG[CT]G
SmaI 401 CCCGGG CCC^GGG
TfiI 1137 GATTC G^A[AT]TC
Tru9I 146 TTAA T^TAA
Tth111I 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 61 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1601 CCATGCTGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 401 CCCGGG C^CCGGG
XmaI 401 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 509 GGGCCC GGGCC^C
1585 GGGCCC
BalI 1598 TGGCCA TGG^CCA
1637 TGGCCA
BbeI 862 GGCGCC GGCGC^C
1246 GGCGCC
BsaWI 923 ACCGGT [AT]^CCGG[AT]
1159 ACCGGA
BsmAI 43 GAGAC GTCTC(1/5)
320 GAGAC
BspMI 1093 ACCTGC ACCTGC(4/8)
1553 ACCTGC
BsrBI 1209 CCGCTC CCGCTC(-3/-3)
1652 CCGCTC
BstAPI 612 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1013 GCACTGACTGC
BstYI 287 GGATCC [GA]^GATC[CT]
1571 AGATCC
EagI 723 CGGCCG C^GGCCG
1035 CGGCCG
EclXI 723 CGGCCG C^GGCCG
1035 CGGCCG
Eco52I 723 CGGCCG C^GGCCG
1035 CGGCCG
EcoT14I 642 CCTTGG C^C[AT][AT]GG
1589 CCAAGG
KasI 862 GGCGCC G^GCGCC
1246 GGCGCC
MflI 287 GGATCC [GA]^GATC[CT]
1571 AGATCC
MluNI 1598 TGGCCA TGG^CCA
1637 TGGCCA
MscI 1598 TGGCCA TGG^CCA
1637 TGGCCA
MslI 144 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1602 CATGCTGATG
MspA1I 951 CAGCTG C[AC]G^C[GT]G
1185 CCGCTG
NarI 862 GGCGCC GG^CGCC
1246 GGCGCC
SacI 729 GAGCTC GAGCT^C
1293 GAGCTC
SfoI 862 GGCGCC GGC^GCC
1246 GGCGCC
SmlI 200 CTCAAG C^T[CT][GA]AG
515 CTCAAG
StuI 499 AGGCCT AGG^CCT
536 AGGCCT
StyI 642 CCTTGG C^C[AT][AT]GG
1589 CCAAGG
XhoII 287 GGATCC [GA]^GATC[CT]
1571 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Aor51HI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIV PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I XmnI