Restriction map for pF1KB7861
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AhdI        AlwNI       AocI        ApoI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaBI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FspI        HincII      
HindII      HindIII     HpaI        KspI        MamI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        111  GGTACC               G^GTACC
AflIII       1175  ACGTGT               A^C[GA][CT]GT
AgeI          923  ACCGGT               A^CCGGT
Alw44I        588  GTGCAC               G^TGCAC
ApaLI         588  GTGCAC               G^TGCAC
Asp718I       111  GGTACC               G^GTACC
AspI         1216  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         287  GGATCC               G^GATCC
BfaI         1303  CTAG                 C^TAG
BlpI          763  GCTGAGC              GC^T[ACGT]AGC
BpmI          546  CTCCAG               CTGGAG(16/14)
Bpu1102I      763  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1174  TACGTG               [CT]AC^GT[GA]
BsaI          320  GAGACC               GGTCTC(1/5)
BssHII        704  GCGCGC               G^CGCGC
BssSI        1564  CACGAG               CACGAG(-5/-1)
BstEII       1221  GGTCACC              G^GT[ACGT]ACC
BstPI        1221  GGTCACC              G^GT[ACGT]ACC
BstXI        1589  CCAAGGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         763  GCTGAGC              GC^T[ACGT]AGC
DraIII        964  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         491  CCTTCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1221  GGTCACC              G^GT[ACGT]ACC
FseI          737  GGCCGGCC             GGCCGG^CC
KpnI          111  GGTACC               GGTAC^C
MaeI         1303  CTAG                 C^TAG
MaeII        1175  ACGT                 A^CGT
MseI          146  TTAA                 T^TAA
NlaIII       1602  CATG                 CATG^
PflFI        1216  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          61  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         923  ACCGGT               A^CCGGT
PshAI         799  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         951  CAGCTG               CAG^CTG
SexAI         965  ACCTGGT              A^CC[AT]GGT
SgrAI         976  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          401  CCCGGG               CCC^GGG
TfiI         1137  GATTC                G^A[AT]TC
Tru9I         146  TTAA                 T^TAA
Tth111I      1216  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         61  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1601  CCATGCTGATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         401  CCCGGG               C^CCGGG
XmaI          401  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

ApaI          509  GGGCCC               GGGCC^C
             1585  GGGCCC              
BalI         1598  TGGCCA               TGG^CCA
             1637  TGGCCA              
BbeI          862  GGCGCC               GGCGC^C
             1246  GGCGCC              
BsaWI         923  ACCGGT               [AT]^CCGG[AT]
             1159  ACCGGA              
BsmAI          43  GAGAC                GTCTC(1/5)
              320  GAGAC               
BspMI        1093  ACCTGC               ACCTGC(4/8)
             1553  ACCTGC              
BsrBI        1209  CCGCTC               CCGCTC(-3/-3)
             1652  CCGCTC              
BstAPI        612  GCAGAGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1013  GCACTGACTGC         
BstYI         287  GGATCC               [GA]^GATC[CT]
             1571  AGATCC              
EagI          723  CGGCCG               C^GGCCG
             1035  CGGCCG              
EclXI         723  CGGCCG               C^GGCCG
             1035  CGGCCG              
Eco52I        723  CGGCCG               C^GGCCG
             1035  CGGCCG              
EcoT14I       642  CCTTGG               C^C[AT][AT]GG
             1589  CCAAGG              
KasI          862  GGCGCC               G^GCGCC
             1246  GGCGCC              
MflI          287  GGATCC               [GA]^GATC[CT]
             1571  AGATCC              
MluNI        1598  TGGCCA               TGG^CCA
             1637  TGGCCA              
MscI         1598  TGGCCA               TGG^CCA
             1637  TGGCCA              
MslI          144  CATTAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1602  CATGCTGATG          
MspA1I        951  CAGCTG               C[AC]G^C[GT]G
             1185  CCGCTG              
NarI          862  GGCGCC               GG^CGCC
             1246  GGCGCC              
SacI          729  GAGCTC               GAGCT^C
             1293  GAGCTC              
SfoI          862  GGCGCC               GGC^GCC
             1246  GGCGCC              
SmlI          200  CTCAAG               C^T[CT][GA]AG
              515  CTCAAG              
StuI          499  AGGCCT               AGG^CCT
              536  AGGCCT              
StyI          642  CCTTGG               C^C[AT][AT]GG
             1589  CCAAGG              
XhoII         287  GGATCC               [GA]^GATC[CT]
             1571  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        Aor51HI     Asp700I     AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIV       PleI        
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com