################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AhdI AlwNI AocI ApoI AscI AseI AsnI AspEI AviII AvrII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 111 GGTACC G^GTACC AflIII 1175 ACGTGT A^C[GA][CT]GT AgeI 923 ACCGGT A^CCGGT Alw44I 588 GTGCAC G^TGCAC ApaLI 588 GTGCAC G^TGCAC Asp718I 111 GGTACC G^GTACC AspI 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 287 GGATCC G^GATCC BfaI 1303 CTAG C^TAG BlpI 763 GCTGAGC GC^T[ACGT]AGC BpmI 546 CTCCAG CTGGAG(16/14) Bpu1102I 763 GCTGAGC GC^T[ACGT]AGC BsaAI 1174 TACGTG [CT]AC^GT[GA] BsaI 320 GAGACC GGTCTC(1/5) BssHII 704 GCGCGC G^CGCGC BssSI 1564 CACGAG CACGAG(-5/-1) BstEII 1221 GGTCACC G^GT[ACGT]ACC BstPI 1221 GGTCACC G^GT[ACGT]ACC BstXI 1589 CCAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 763 GCTGAGC GC^T[ACGT]AGC DraIII 964 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 491 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1221 GGTCACC G^GT[ACGT]ACC FseI 737 GGCCGGCC GGCCGG^CC KpnI 111 GGTACC GGTAC^C MaeI 1303 CTAG C^TAG MaeII 1175 ACGT A^CGT MseI 146 TTAA T^TAA NlaIII 1602 CATG CATG^ PflFI 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 61 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 923 ACCGGT A^CCGGT PshAI 799 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 951 CAGCTG CAG^CTG SexAI 965 ACCTGGT A^CC[AT]GGT SgrAI 976 CACCGGCG C[GA]^CCGG[CT]G SmaI 401 CCCGGG CCC^GGG TfiI 1137 GATTC G^A[AT]TC Tru9I 146 TTAA T^TAA Tth111I 1216 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 61 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1601 CCATGCTGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 401 CCCGGG C^CCGGG XmaI 401 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### ApaI 509 GGGCCC GGGCC^C 1585 GGGCCC BalI 1598 TGGCCA TGG^CCA 1637 TGGCCA BbeI 862 GGCGCC GGCGC^C 1246 GGCGCC BsaWI 923 ACCGGT [AT]^CCGG[AT] 1159 ACCGGA BsmAI 43 GAGAC GTCTC(1/5) 320 GAGAC BspMI 1093 ACCTGC ACCTGC(4/8) 1553 ACCTGC BsrBI 1209 CCGCTC CCGCTC(-3/-3) 1652 CCGCTC BstAPI 612 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1013 GCACTGACTGC BstYI 287 GGATCC [GA]^GATC[CT] 1571 AGATCC EagI 723 CGGCCG C^GGCCG 1035 CGGCCG EclXI 723 CGGCCG C^GGCCG 1035 CGGCCG Eco52I 723 CGGCCG C^GGCCG 1035 CGGCCG EcoT14I 642 CCTTGG C^C[AT][AT]GG 1589 CCAAGG KasI 862 GGCGCC G^GCGCC 1246 GGCGCC MflI 287 GGATCC [GA]^GATC[CT] 1571 AGATCC MluNI 1598 TGGCCA TGG^CCA 1637 TGGCCA MscI 1598 TGGCCA TGG^CCA 1637 TGGCCA MslI 144 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1602 CATGCTGATG MspA1I 951 CAGCTG C[AC]G^C[GT]G 1185 CCGCTG NarI 862 GGCGCC GG^CGCC 1246 GGCGCC SacI 729 GAGCTC GAGCT^C 1293 GAGCTC SfoI 862 GGCGCC GGC^GCC 1246 GGCGCC SmlI 200 CTCAAG C^T[CT][GA]AG 515 CTCAAG StuI 499 AGGCCT AGG^CCT 536 AGGCCT StyI 642 CCTTGG C^C[AT][AT]GG 1589 CCAAGG XhoII 287 GGATCC [GA]^GATC[CT] 1571 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI Aor51HI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIV PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I XmnI