Restriction map for
pF1KB7850
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AhdI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbsI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsgI BsiWI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PmeI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
StyI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 118 GTCGAC GT^[AC][GT]AC
AgeI 92 ACCGGT A^CCGGT
Alw44I 1522 GTGCAC G^TGCAC
AlwI 57 GATCC GGATC(4/5)
ApaLI 1522 GTGCAC G^TGCAC
AseI 279 ATTAAT AT^TAAT
AsnI 279 ATTAAT AT^TAAT
BanI 523 GGCACC G^G[CT][GA]CC
BglII 1500 AGATCT A^GATCT
BsaI 1795 GAGACC GGTCTC(1/5)
BsaJI 1763 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 92 ACCGGT [AT]^CCGG[AT]
BsiEI 63 CGACCG CG[GA][CT]^CG
BsmBI 13 GAGACG CGTCTC(1/5)
BsmFI 1211 GGGAC GGGAC(10/14)
BspMI 1554 GCAGGT ACCTGC(4/8)
BsrFI 92 ACCGGT [GA]^CCGG[CT]
BssSI 717 CACGAG CACGAG(-5/-1)
BstAPI 1744 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1040 TTCGAA TT^CGAA
BstF5I 1495 CATCC GGATG(2/0)
BstXI 527 CCATTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1500 AGATCT [GA]^GATC[CT]
Cac8I 1129 GCATGC GC[ACGT]^[ACGT]GC
CfoI 902 GCGC GCG^C
Cfr10I 92 ACCGGT [GA]^CCGG[CT]
EarI 10 GAAGAG CTCTTC(1/4)
EcoRI 1586 GAATTC G^AATTC
EcoRV 887 GATATC GAT^ATC
FokI 1495 CATCC GGATG(9/13)
HapII 93 CCGG C^CGG
HgaI 1341 GCGTC GACGC(5/10)
HhaI 902 GCGC GCG^C
HinP1I 902 GCGC G^CGC
HincII 118 GTCGAC GT[CT]^[GA]AC
HindII 118 GTCGAC GT[CT]^[GA]AC
HpaII 93 CCGG C^CGG
Ksp632I 10 GAAGAG CTCTTC(1/4)
MflI 1500 AGATCT [GA]^GATC[CT]
MspI 93 CCGG C^CGG
NspV 1040 TTCGAA TT^CGAA
PinAI 92 ACCGGT A^CCGGT
PshBI 279 ATTAAT AT^TAAT
PstI 479 CTGCAG CTGCA^G
SalI 118 GTCGAC G^TCGAC
ScaI 992 AGTACT AGT^ACT
SfcI 479 CTGCAG C^T[GA][CT]AG
SfuI 1040 TTCGAA TT^CGAA
SphI 1129 GCATGC GCATG^C
SspI 1330 AATATT AAT^ATT
SwaI 807 ATTTAAAT ATTT^AAAT
XcmI 1799 CCAAGCCAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1500 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 488 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1745 CAGATGCTG
BbrPI 1376 CACGTG CAC^GTG
1696 CACGTG
BbvCI 1753 GCTGAGG CCTCAGC(-5/-2)
1783 GCTGAGG
BsaAI 1376 CACGTG [CT]AC^GT[GA]
1696 CACGTG
BseRI 1621 GAGGAG GAGGAG(10/8)
1723 GAGGAG
BsmAI 13 GAGAC GTCTC(1/5)
1795 GAGAC
BsmI 22 GAATGC GAATGC(1/-1)
1177 GAATGC
BspHI 1083 TCATGA T^CATGA
1712 TCATGA
BspLU11I 765 ACATGT A^CATGT
1614 ACATGT
BsrDI 470 CATTGC GCAATG(2/0)
1445 CATTGC
DraI 808 TTTAAA TTT^AAA
1027 TTTAAA
MaeII 1377 ACGT A^CGT
1697 ACGT
MspA1I 757 CAGCTG C[AC]G^C[GT]G
1206 CAGCTG
PmaCI 1376 CACGTG CAC^GTG
1696 CACGTG
PmlI 1376 CACGTG CAC^GTG
1696 CACGTG
PvuII 757 CAGCTG CAG^CTG
1206 CAGCTG
RcaI 1083 TCATGA T^CATGA
1712 TCATGA
XbaI 1572 TCTAGA T^CTAGA
1677 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
Asp700I AspHI AvaII BbvI BfaI
BmyI BpmI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI