Restriction map for
pF1KE1080
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII BalI BamHI BbeI
BbrPI BglI BglII BlpI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstUI CelII
Cfr10I CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI FspI HaeII HindIII
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 1852 GTATAC GT^[AC][GT]AC
AclI 3641 AACGTT AA^CGTT
AcyI 2299 GACGCC G[GA]^CG[CT]C
AflII 3630 CTTAAG C^TTAAG
Alw44I 3525 GTGCAC G^TGCAC
AocI 3585 CCTCAGG CC^T[ACGT]AGG
ApaLI 3525 GTGCAC G^TGCAC
AseI 1245 ATTAAT AT^TAAT
AsnI 1245 ATTAAT AT^TAAT
AspI 2512 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1376 CTCGGG C^[CT]CG[GA]G
BanI 1206 GGCACC G^G[CT][GA]CC
BanII 2381 GAGCTC G[GA]GC[CT]^C
BciVI 1808 GTATCC GTATCC(6/5)
BcnI 45 CCGGG CC^[GC]GG
BfrI 3630 CTTAAG C^TTAAG
BsaAI 1964 CACGTA [CT]AC^GT[GA]
BsaBI 3136 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2299 GACGCC G[GA]^CG[CT]C
BsgI 620 CTGCAC GTGCAG(16/14)
BsoBI 1376 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1620 TGTACA T^GTACA
BspDI 610 ATCGAT AT^CGAT
BspHI 3356 TCATGA T^CATGA
BspMI 733 ACCTGC ACCTGC(4/8)
BsrGI 1620 TGTACA T^GTACA
Bst1107I 1852 GTATAC GTA^TAC
BstEII 79 GGTCACC G^GT[ACGT]ACC
BstPI 79 GGTCACC G^GT[ACGT]ACC
BstXI 1662 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1852 GTATAC GTA^TAC
Bsu36I 3585 CCTCAGG CC^T[ACGT]AGG
ClaI 610 ATCGAT AT^CGAT
Eco81I 3585 CCTCAGG CC^T[ACGT]AGG
EcoO65I 79 GGTCACC G^GT[ACGT]ACC
EcoRV 2644 GATATC GAT^ATC
HapII 45 CCGG C^CGG
HgaI 2299 GACGC GACGC(5/10)
Hin1I 2299 GACGCC G[GA]^CG[CT]C
HpaII 45 CCGG C^CGG
MamI 3136 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2603 CAATTG C^AATTG
MspI 45 CCGG C^CGG
MunI 2603 CAATTG C^AATTG
NciI 45 CCGGG CC^[GC]GG
NcoI 126 CCATGG C^CATGG
NdeI 227 CATATG CA^TATG
PflFI 2512 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1235 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1245 ATTAAT AT^TAAT
Psp1406I 3641 AACGTT AA^CGTT
RcaI 3356 TCATGA T^CATGA
SacI 2381 GAGCTC GAGCT^C
SspBI 1620 TGTACA T^GTACA
Tsp45I 80 GTCAC ^GT[GC]AC
Tth111I 2512 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2779 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvrII 544 CCTAGG C^CTAGG
2209 CCTAGG
BbvCI 3746 CCTCAGC CCTCAGC(-5/-2)
658 GCTGAGG
BcgI 612 CGATGAGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
632 CGAAGGAGCTGC
BclI 1926 TGATCA T^GATCA
2931 TGATCA
BlnI 544 CCTAGG C^CTAGG
2209 CCTAGG
BpmI 2830 CTCCAG CTGGAG(16/14)
336 CTGGAG
BsmI 1563 GAATGC GAATGC(1/-1)
3682 GAATGC
CfoI 33 GCGC GCG^C
1404 GCGC
DsaI 126 CCATGG C^C[GA][CT]GG
553 CCACGG
FauI 259 CCCGC CCCGC(4/6)
3552 GCGGG
FbaI 1926 TGATCA T^GATCA
2931 TGATCA
HhaI 33 GCGC GCG^C
1404 GCGC
HinP1I 33 GCGC G^CGC
1404 GCGC
HpaI 1822 GTTAAC GTT^AAC
3291 GTTAAC
MslI 1612 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1777 CACATCAGTG
MspA1I 1685 CAGCTG C[AC]G^C[GT]G
3557 CAGCTG
PpuMI 1665 AGGTCCT [GA]G^G[AT]CC[CT]
3757 AGGACCC
PvuII 1685 CAGCTG CAG^CTG
3557 CAGCTG
SapI 1519 GCTCTTC GCTCTTC(1/4)
3701 GCTCTTC
SmlI 2413 CTCAAG C^T[CT][GA]AG
3630 CTTAAG
TaqI 611 TCGA T^CGA
3330 TCGA
TthHB8I 611 TCGA T^CGA
3330 TCGA
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp509I TspRI XcmI XhoII XmnI