Restriction map for
pF1KB7208
##################################
# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AgeI AhdI AluI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AfaI 225 GTAC GT^AC
AflIII 28 ACACGT A^C[GA][CT]GT
AlwI 42 GGATC GGATC(4/5)
AlwNI 76 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 228 CCCGGG C^[CT]CG[GA]G
BbrPI 29 CACGTG CAC^GTG
BbvI 81 GCTGC GCAGC(8/12)
BfaI 251 CTAG C^TAG
BsaAI 29 CACGTG [CT]AC^GT[GA]
BsaJI 228 CCCGGG C^C[ACGT][ACGT]GG
BsgI 32 GTGCAG GTGCAG(16/14)
BsiYI 17 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 17 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 13 GTCCC GGGAC(10/14)
BsoBI 228 CCCGGG C^[CT]CG[GA]G
BspHI 98 TCATGA T^CATGA
BsrI 286 ACTGG ACTGG(1/-1)
BstAPI 144 GCATAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 55 CATCC GGATG(2/0)
BstNI 23 CCAGG CC^[AT]GG
Cac8I 200 GCTTGC GC[ACGT]^[ACGT]GC
EarI 242 CTCTTC CTCTTC(1/4)
EcoRII 23 CCAGG ^CC[AT]GG
Fnu4HI 81 GCTGC GC^[ACGT]GC
FokI 55 CATCC GGATG(9/13)
HapII 229 CCGG C^CGG
HgaI 6 GACGC GACGC(5/10)
HinfI 240 GACTC G^A[ACGT]TC
HpaII 229 CCGG C^CGG
HphI 232 GGTGA GGTGA(8/7)
ItaI 81 GCTGC GC^[ACGT]GC
Ksp632I 242 CTCTTC CTCTTC(1/4)
MaeI 251 CTAG C^TAG
MaeII 30 ACGT A^CGT
MaeIII 238 GTGAC ^GT[ACGT]AC
MboII 243 TCTTC GAAGA(8/7)
MseI 134 TTAA T^TAA
MslI 261 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 229 CCGG C^CGG
MvaI 23 CCAGG CC^[AT]GG
MwoI 144 GCATAGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 99 CATG CATG^
PleI 240 GACTC GAGTC(4/5)
PmaCI 29 CACGTG CAC^GTG
PmlI 29 CACGTG CAC^GTG
PshAI 6 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 98 TCATGA T^CATGA
RsaI 225 GTAC GT^AC
SfaNI 95 GCATC GCATC(5/9)
SmaI 228 CCCGGG CCC^GGG
SspI 136 AATATT AAT^ATT
TaqI 298 TCGA T^CGA
Tru9I 134 TTAA T^TAA
TseI 81 GCTGC G^C[AT]GC
Tsp45I 238 GTGAC ^GT[GC]AC
TthHB8I 298 TCGA T^CGA
XmaCI 228 CCCGGG C^CCGGG
XmaI 228 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BcnI 228 CCCGG CC^[GC]GG
229 CCGGG
Cfr13I 254 GGCCC G^G[ACGT]CC
302 GGCCC
DdeI 124 CTAAG C^T[ACGT]AG
291 CTCAG
DpnII 43 GATC ^GATC
308 GATC
HaeIII 254 GGCC GG^CC
302 GGCC
MboI 43 GATC ^GATC
308 GATC
MnlI 91 GAGG CCTC(7/6)
300 GAGG
NciI 228 CCCGG CC^[GC]GG
229 CCGGG
NdeII 43 GATC ^GATC
308 GATC
Sau3AI 43 GATC ^GATC
308 GATC
Sau96I 254 GGCCC G^G[ACGT]CC
302 GGCCC
TspRI 285 CACTG CA[GC]TG[ACGT][ACGT]^
293 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
BssKI ScrFI