##################################
# NO CUT ENZYMES ( 198 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DpnII DraII DraIII DrdI DsaI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
TaqI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
BfaI 206 CTAG C^TAG
BsaAI 299 CACGTA [CT]AC^GT[GA]
BsaJI 23 CCCCGG C^C[ACGT][ACGT]GG
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsmFI 21 GTCCC GGGAC(10/14)
Bsp1407I 285 TGTACA T^GTACA
BsrGI 285 TGTACA T^GTACA
BstNI 33 CCAGG CC^[AT]GG
Cac8I 4 GCTTGC GC[ACGT]^[ACGT]GC
DraI 66 TTTAAA TTT^AAA
EaeI 26 CGGCCG [CT]^GGCC[GA]
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoRII 33 CCAGG ^CC[AT]GG
HaeIII 27 GGCC GG^CC
MaeI 206 CTAG C^TAG
MvaI 33 CCAGG CC^[AT]GG
MwoI 4 GCTTGCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI 242 GAGTC GAGTC(4/5)
PshAI 376 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmlI 184 CTTGAG C^T[CT][GA]AG
SspBI 285 TGTACA T^GTACA
SwaI 65 ATTTAAAT ATTT^AAAT
XbaI 205 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 15 CGCG CG^CG
17 CGCG
AciI 14 CCGC CCGC(-3/-1)
18 GCGG
AfaI 201 GTAC GT^AC
286 GTAC
AluI 135 AGCT AG^CT
254 AGCT
AvaII 20 GGTCC G^G[AT]CC
249 GGTCC
BbsI 78 GAAGAC GAAGAC(2/6)
383 GTCTTC
BbvI 322 GCAGC GCAGC(8/12)
10 GCTGC
BcnI 24 CCCGG CC^[GC]GG
246 CCGGG
BpuAI 78 GAAGAC GAAGAC(2/6)
383 GTCTTC
BstUI 15 CGCG CG^CG
17 CGCG
CfoI 8 GCGC GCG^C
16 GCGC
Cfr13I 20 GGTCC G^G[ACGT]CC
249 GGTCC
HapII 25 CCGG C^CGG
246 CCGG
HhaI 8 GCGC GCG^C
16 GCGC
HinP1I 8 GCGC G^CGC
16 GCGC
HpaII 25 CCGG C^CGG
246 CCGG
MaeII 176 ACGT A^CGT
300 ACGT
MseI 67 TTAA T^TAA
173 TTAA
MspI 25 CCGG C^CGG
246 CCGG
MvnI 15 CGCG CG^CG
17 CGCG
NciI 24 CCCGG CC^[GC]GG
246 CCGGG
RsaI 201 GTAC GT^AC
286 GTAC
Sau96I 20 GGTCC G^G[ACGT]CC
249 GGTCC
SfcI 131 CTACAG C^T[GA][CT]AG
137 CTACAG
Tru9I 67 TTAA T^TAA
173 TTAA
TseI 10 GCTGC G^C[AT]GC
322 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
BssKI Fnu4HI HinfI ItaI MboII
MnlI NlaIV ScrFI TfiI