Restriction map for
pF1KB6457
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AvrII BalI BamHI BanI
BbeI BbrPI BbsI BcgI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FseI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeIII MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 795 GTCTAC GT^[AC][GT]AC
AclI 38 AACGTT AA^CGTT
AcsI 690 GAATTC [GA]^AATT[CT]
AlwI 70 GATCC GGATC(4/5)
AocI 400 CCTGAGG CC^T[ACGT]AGG
ApoI 690 GAATTC [GA]^AATT[CT]
Asp700I 497 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 413 GAGCAC G[AT]GC[AT]^C
AviII 90 TGCGCA TGC^GCA
BbvCI 695 CCTCAGC CCTCAGC(-5/-2)
BciVI 201 GTATCC GTATCC(6/5)
BclI 846 TGATCA T^GATCA
BcnI 765 CCGGG CC^[GC]GG
BsaBI 870 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4 GAGACC GGTCTC(1/5)
BseRI 164 GAGGAG GAGGAG(10/8)
BsiHKAI 413 GAGCAC G[AT]GC[AT]^C
BsiWI 677 CGTACG C^GTACG
BstXI 527 CCAAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 69 AGATCC [GA]^GATC[CT]
Bsu36I 400 CCTGAGG CC^T[ACGT]AGG
DraI 486 TTTAAA TTT^AAA
EaeI 665 CGGCCA [CT]^GGCC[GA]
Eco81I 400 CCTGAGG CC^T[ACGT]AGG
EcoRI 690 GAATTC G^AATTC
FauI 8 CCCGC CCCGC(4/6)
FbaI 846 TGATCA T^GATCA
FspI 90 TGCGCA TGC^GCA
HaeII 761 AGCGCC [GA]GCGC^[CT]
HindIII 549 AAGCTT A^AGCTT
HphI 802 TCACC GGTGA(8/7)
MaeII 39 ACGT A^CGT
MamI 870 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 69 AGATCC [GA]^GATC[CT]
MslI 82 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 860 CAGCTG C[AC]G^C[GT]G
NciI 765 CCGGG CC^[GC]GG
Psp1406I 38 AACGTT AA^CGTT
PvuII 860 CAGCTG CAG^CTG
SapI 410 GAAGAGC GCTCTTC(1/4)
ScaI 186 AGTACT AGT^ACT
SfcI 728 CTGTAG C^T[GA][CT]AG
TfiI 502 GATTC G^A[AT]TC
TspRI 843 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 387 TCTAGA T^CTAGA
XcmI 840 CCACACTGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 69 AGATCC [GA]^GATC[CT]
XmnI 497 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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ApaI 139 GGGCCC GGGCC^C
767 GGGCCC
BfaI 388 CTAG C^TAG
539 CTAG
BsaJI 654 CCAGGG C^C[ACGT][ACGT]GG
816 CCAGGG
BsmAI 4 GAGAC GTCTC(1/5)
833 GAGAC
BsrI 534 ACTGG ACTGG(1/-1)
668 CCAGT
BstNI 654 CCAGG CC^[AT]GG
816 CCAGG
CfoI 91 GCGC GCG^C
762 GCGC
DraII 138 AGGGCCC [GA]G^G[ACGT]CC[CT]
139 GGGCCCC
EarI 21 GAAGAG CTCTTC(1/4)
410 GAAGAG
EcoO109I 138 AGGGCCC [GA]G^G[ACGT]CC[CT]
139 GGGCCCC
EcoRII 654 CCAGG ^CC[AT]GG
816 CCAGG
HapII 28 CCGG C^CGG
765 CCGG
HhaI 91 GCGC GCG^C
762 GCGC
HinP1I 91 GCGC G^CGC
762 GCGC
HpaII 28 CCGG C^CGG
765 CCGG
Ksp632I 21 GAAGAG CTCTTC(1/4)
410 GAAGAG
MaeI 388 CTAG C^TAG
539 CTAG
MboII 21 GAAGA GAAGA(8/7)
410 GAAGA
MspI 28 CCGG C^CGG
765 CCGG
MvaI 654 CCAGG CC^[AT]GG
816 CCAGG
SfaNI 278 GCATC GCATC(5/9)
703 GCATC
TaqI 246 TCGA T^CGA
756 TCGA
TthHB8I 246 TCGA T^CGA
756 TCGA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BmyI BsiYI BslI Bsp1286I BssKI
BstF5I Cfr13I DdeI DpnII Fnu4HI
FokI HaeIII HinfI ItaI MboI
MnlI MseI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp509I