##################################
# NO CUT ENZYMES (  172 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FokI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HinfI       HpaI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfaNI       SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        377  GGTACC               G^GTACC
AcyI           39  GGCGCC               G[GA]^CG[CT]C
AlwI          330  GGATC                GGATC(4/5)
ApaI          147  GGGCCC               GGGCC^C
Asp718I       377  GGTACC               G^GTACC
BbeI           39  GGCGCC               GGCGC^C
BsaHI          39  GGCGCC               G[GA]^CG[CT]C
BsaI           88  GGTCTC               GGTCTC(1/5)
BsmAI          89  GTCTC                GTCTC(1/5)
BsrFI         103  GCCGGC               [GA]^CCGG[CT]
BssSI         120  CTCGTG               CACGAG(-5/-1)
BstXI         362  CCACTACCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         330  GGATCT               [GA]^GATC[CT]
CfoI           40  GCGC                 GCG^C
Cfr10I        103  GCCGGC               [GA]^CCGG[CT]
DdeI          171  CTCAG                C^T[ACGT]AG
DpnII         331  GATC                 ^GATC
DraII         147  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        136  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          395  TGGCCG               [CT]^GGCC[GA]
EcoO109I      147  GGGCCCT              [GA]G^G[ACGT]CC[CT]
HaeII          39  GGCGCC               [GA]GCGC^[CT]
HapII         104  CCGG                 C^CGG
HhaI           40  GCGC                 GCG^C
Hin1I          39  GGCGCC               G[GA]^CG[CT]C
HinP1I         40  GCGC                 G^CGC
HpaII         104  CCGG                 C^CGG
HphI          384  GGTGA                GGTGA(8/7)
KasI           39  GGCGCC               G^GCGCC
KpnI          377  GGTACC               GGTAC^C
MboI          331  GATC                 ^GATC
MboII         293  TCTTC                GAAGA(8/7)
MflI          330  GGATCT               [GA]^GATC[CT]
MslI          363  CACTACCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          104  CCGG                 C^CGG
NaeI          103  GCCGGC               GCC^GGC
NarI           39  GGCGCC               GG^CGCC
NdeII         331  GATC                 ^GATC
NgoMIV        103  GCCGGC               G^CCGGC
PstI          109  CTGCAG               CTGCA^G
PvuII         262  CAGCTG               CAG^CTG
Sau3AI        331  GATC                 ^GATC
SfcI          109  CTGCAG               C^T[GA][CT]AG
SfiI          423  GGCCTGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           39  GGCGCC               GGC^GCC
XcmI          474  CCATGGCTGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         330  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AluI          209  AGCT                 AG^CT
              263  AGCT                
BglI          117  GCCCTCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              424  GCCTGGCTGGC         
BsmFI         467  GGGAC                GGGAC(10/14)
              489  GGGAC               
SexAI         194  ACCTGGT              A^CC[AT]GGT
              380  ACCAGGT             


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AfaI        BanI        BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DsaI        EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      ItaI        MnlI        MspA1I      MvaI        
MwoI        NcoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       StyI        TseI        TspRI