Restriction map for
pF1KB7646
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
CpoI DraI DraII DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI StyI
SwaI TseI Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 526 TCCGGA T^CCGGA
AhdI 1146 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1146 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 754 GAGCTC G[AT]GC[AT]^C
BanII 754 GAGCTC G[GA]GC[CT]^C
BbvCI 1116 GCTGAGG CCTCAGC(-5/-2)
BciVI 529 GGATAC GTATCC(6/5)
BfaI 275 CTAG C^TAG
BglII 99 AGATCT A^GATCT
BpmI 416 CTCCAG CTGGAG(16/14)
BsaAI 65 TACGTG [CT]AC^GT[GA]
BsaWI 526 TCCGGA [AT]^CCGG[AT]
BseAI 526 TCCGGA T^CCGGA
BsiHKAI 754 GAGCTC G[AT]GC[AT]^C
BspDI 940 ATCGAT AT^CGAT
BspEI 526 TCCGGA T^CCGGA
BspHI 389 TCATGA T^CATGA
BsrFI 1150 GCCGGT [GA]^CCGG[CT]
Cfr10I 1150 GCCGGT [GA]^CCGG[CT]
ClaI 940 ATCGAT AT^CGAT
DraIII 572 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1063 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1146 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 811 GAAGAG CTCTTC(1/4)
EcoRV 937 GATATC GAT^ATC
HaeIII 470 GGCC GG^CC
HphI 798 TCACC GGTGA(8/7)
Ksp632I 811 GAAGAG CTCTTC(1/4)
MaeI 275 CTAG C^TAG
MroI 526 TCCGGA T^CCGGA
MspA1I 1114 CAGCTG C[AC]G^C[GT]G
NdeI 122 CATATG CA^TATG
PvuII 1114 CAGCTG CAG^CTG
RcaI 389 TCATGA T^CATGA
SacI 754 GAGCTC GAGCT^C
SexAI 573 ACCTGGT A^CC[AT]GGT
SpeI 274 ACTAGT A^CTAGT
SphI 497 GCATGC GCATG^C
SspI 205 AATATT AAT^ATT
TaqI 941 TCGA T^CGA
TthHB8I 941 TCGA T^CGA
XcmI 211 CCAATATGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 981 GATCC GGATC(4/5)
738 GGATC
AlwNI 802 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
979 CAGATCCTG
AvaII 580 GGTCC G^G[AT]CC
640 GGTCC
BsaI 53 GAGACC GGTCTC(1/5)
1153 GGTCTC
BsmAI 53 GAGAC GTCTC(1/5)
1154 GTCTC
BsmFI 641 GTCCC GGGAC(10/14)
1098 GTCCC
BstYI 99 AGATCT [GA]^GATC[CT]
980 AGATCC
Cac8I 17 GCCAGC GC[ACGT]^[ACGT]GC
497 GCATGC
HapII 527 CCGG C^CGG
1151 CCGG
HpaII 527 CCGG C^CGG
1151 CCGG
MaeII 66 ACGT A^CGT
1089 ACGT
MflI 99 AGATCT [GA]^GATC[CT]
980 AGATCC
MslI 304 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
390 CATGATTGTG
MspI 527 CCGG C^CGG
1151 CCGG
MwoI 492 GCCAGGCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
656 GCACAATCAGC
NlaIV 468 GGGGCC GG[ACGT]^[ACGT]CC
640 GGTCCC
PflMI 571 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
801 CCAGAAACTGG
SfaNI 310 GATGC GCATC(5/9)
442 GCATC
SmlI 22 CTCAAG C^T[CT][GA]AG
1044 CTTGAG
Van91I 571 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
801 CCAGAAACTGG
XhoII 99 AGATCT [GA]^GATC[CT]
980 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI BbsI BmyI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstEII BstF5I BstNI
BstPI Cfr13I DdeI DpnII EcoNI
EcoO65I EcoRII FokI HinfI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I ScrFI TfiI Tru9I Tsp45I
TspRI