Restriction map for
pF1KB8647
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BbrPI
BcgI BciVI BfrI BsaI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrBI BsrDI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eco47III Eco81I EcoRV HindIII
HpaI KpnI KspI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII ScaI SfuI SgrAI
SnaBI SpeI SspI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 618 GAATTC [GA]^AATT[CT]
AflIII 2170 ACATGT A^C[GA][CT]GT
AhdI 1376 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 618 GAATTC [GA]^AATT[CT]
AspEI 1376 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1208 TGCGCA TGC^GCA
AvrII 198 CCTAGG C^CTAGG
BbsI 964 GTCTTC GAAGAC(2/6)
BbvCI 1023 CCTCAGC CCTCAGC(-5/-2)
BclI 2444 TGATCA T^GATCA
BglII 1283 AGATCT A^GATCT
BlnI 198 CCTAGG C^CTAGG
BlpI 468 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
BpuAI 964 GTCTTC GAAGAC(2/6)
BsaAI 649 TACGTG [CT]AC^GT[GA]
BsaBI 1260 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1592 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1325 TGTACA T^GTACA
BspLU11I 2170 ACATGT A^CATGT
BsrGI 1325 TGTACA T^GTACA
BssHII 2044 GCGCGC G^CGCGC
BssSI 160 CTCGTG CACGAG(-5/-1)
BstAPI 486 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2466 GGTGACC G^GT[ACGT]ACC
BstPI 2466 GGTGACC G^GT[ACGT]ACC
CelII 468 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1376 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1872 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2466 GGTGACC G^GT[ACGT]ACC
EcoRI 618 GAATTC G^AATTC
EcoT22I 1479 ATGCAT ATGCA^T
FbaI 2444 TGATCA T^GATCA
FseI 2401 GGCCGGCC GGCCGG^CC
FspI 1208 TGCGCA TGC^GCA
MamI 1260 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1738 CCATGG C^CATGG
NsiI 1479 ATGCAT ATGCA^T
SacI 343 GAGCTC GAGCT^C
SalI 1766 GTCGAC G^TCGAC
SapI 1537 GAAGAGC GCTCTTC(1/4)
SbfI 717 CCTGCAGG CCTGCA^GG
SexAI 2255 ACCAGGT A^CC[AT]GGT
SfiI 1678 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 113 CCCGGG CCC^GGG
SmlI 337 CTCAAG C^T[CT][GA]AG
SphI 2270 GCATGC GCATG^C
Sse8387I 717 CCTGCAGG CCTGCA^GG
SspBI 1325 TGTACA T^GTACA
TfiI 292 GAATC G^A[AT]TC
Tsp509I 619 AATT ^AATT
XmaCI 113 CCCGGG C^CCGGG
XmaI 113 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1766 GTCGAC GT^[AC][GT]AC
2422 GTCTAC
Alw44I 1161 GTGCAC G^TGCAC
1312 GTGCAC
AlwNI 487 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
2227 CAGGACCTG
ApaLI 1161 GTGCAC G^TGCAC
1312 GTGCAC
BamHI 1235 GGATCC G^GATCC
2453 GGATCC
BbeI 2095 GGCGCC GGCGC^C
2110 GGCGCC
BsmAI 2167 GAGAC GTCTC(1/5)
1520 GTCTC
BspMI 418 ACCTGC ACCTGC(4/8)
716 ACCTGC
BstXI 21 CCAGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1415 CCAACGGCCTGG
DraIII 1999 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
2348 CACTCAGTG
EagI 1253 CGGCCG C^GGCCG
2015 CGGCCG
EclXI 1253 CGGCCG C^GGCCG
2015 CGGCCG
Eco52I 1253 CGGCCG C^GGCCG
2015 CGGCCG
HincII 520 GTCAAC GT[CT]^[GA]AC
1766 GTCGAC
HindII 520 GTCAAC GT[CT]^[GA]AC
1766 GTCGAC
KasI 2095 GGCGCC G^GCGCC
2110 GGCGCC
NarI 2095 GGCGCC GG^CGCC
2110 GGCGCC
PflMI 1998 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2457 CCAGCAAGTGG
SfoI 2095 GGCGCC GGC^GCC
2110 GGCGCC
StuI 86 AGGCCT AGG^CCT
971 AGGCCT
Van91I 1998 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2457 CCAGCAAGTGG
XcmI 353 CCAGCACCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
791 CCATGTATGACGTGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII