Restriction map for
pF1KE0008
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1329 GTCTAC GT^[AC][GT]AC
AflIII 1305 ACATGT A^C[GA][CT]GT
AhdI 791 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 740 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1675 ATTAAT AT^TAAT
AsnI 1675 ATTAAT AT^TAAT
AspEI 791 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 408 GTGCTC G[AT]GC[AT]^C
AvaII 1474 GGACC G^G[AT]CC
BalI 1904 TGGCCA TGG^CCA
BcnI 1613 CCCGG CC^[GC]GG
BpmI 478 CTGGAG CTGGAG(16/14)
BsaI 1381 GAGACC GGTCTC(1/5)
BsiHKAI 408 GTGCTC G[AT]GC[AT]^C
BsmFI 1824 GGGAC GGGAC(10/14)
Bsp1407I 1308 TGTACA T^GTACA
BspLU11I 1305 ACATGT A^CATGT
BspMI 1543 GCAGGT ACCTGC(4/8)
BsrGI 1308 TGTACA T^GTACA
BstYI 1991 GGATCT [GA]^GATC[CT]
CfoI 1047 GCGC GCG^C
DraI 340 TTTAAA TTT^AAA
EaeI 1904 TGGCCA [CT]^GGCC[GA]
Eam1105I 791 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1195 CTCTTC CTCTTC(1/4)
EcoT22I 85 ATGCAT ATGCA^T
FauI 1743 CCCGC CCCGC(4/6)
HapII 1614 CCGG C^CGG
HgaI 1826 GACGC GACGC(5/10)
HhaI 1047 GCGC GCG^C
HinP1I 1047 GCGC G^CGC
HindIII 1445 AAGCTT A^AGCTT
HpaII 1614 CCGG C^CGG
Ksp632I 1195 CTCTTC CTCTTC(1/4)
MflI 1991 GGATCT [GA]^GATC[CT]
MluNI 1904 TGGCCA TGG^CCA
MscI 1904 TGGCCA TGG^CCA
MspI 1614 CCGG C^CGG
NciI 1613 CCCGG CC^[GC]GG
NdeI 1372 CATATG CA^TATG
NsiI 85 ATGCAT ATGCA^T
PshBI 1675 ATTAAT AT^TAAT
PstI 1266 CTGCAG CTGCA^G
SapI 1194 GCTCTTC GCTCTTC(1/4)
SfcI 1266 CTGCAG C^T[GA][CT]AG
SmlI 1146 CTTGAG C^T[CT][GA]AG
SphI 1337 GCATGC GCATG^C
SspBI 1308 TGTACA T^GTACA
SspI 371 AATATT AAT^ATT
TaqI 1454 TCGA T^CGA
TthHB8I 1454 TCGA T^CGA
XhoII 1991 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwI 706 GATCC GGATC(4/5)
1991 GGATC
Asp700I 588 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
788 GAAGACTTTC
BanII 1192 GGGCTC G[GA]GC[CT]^C
1535 GGGCTC
BbsI 788 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BbvI 265 GCAGC GCAGC(8/12)
1125 GCAGC
BpuAI 788 GAAGAC GAAGAC(2/6)
1088 GTCTTC
BsaAI 157 TACGTA [CT]AC^GT[GA]
1488 TACGTA
BsrDI 498 CATTGC GCAATG(2/0)
46 GCAATG
BstNI 886 CCTGG CC^[AT]GG
1121 CCTGG
Cfr13I 449 GGCCC G^G[ACGT]CC
1474 GGACC
EcoRII 886 CCTGG ^CC[AT]GG
1121 CCTGG
Fnu4HI 265 GCAGC GC^[ACGT]GC
1125 GCAGC
ItaI 265 GCAGC GC^[ACGT]GC
1125 GCAGC
MaeII 158 ACGT A^CGT
1489 ACGT
MslI 507 CACAACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1513 CACACCCATG
MvaI 886 CCTGG CC^[AT]GG
1121 CCTGG
MwoI 1049 GCCATTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1284 GCCAACACTGC
NcoI 1181 CCATGG C^CATGG
1518 CCATGG
NlaIV 1474 GGACCC GG[ACGT]^[ACGT]CC
1600 GGTTCC
Sau96I 449 GGCCC G^G[ACGT]CC
1474 GGACC
SfaNI 1044 GATGC GCATC(5/9)
1033 GCATC
SnaBI 157 TACGTA TAC^GTA
1488 TACGTA
TseI 265 GCAGC G^C[AT]GC
1125 GCAGC
XmnI 588 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
788 GAAGACTTTC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AciI AcsI AfaI AluI ApoI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I Cac8I DdeI DpnII DsaI
EcoT14I FokI HaeIII HinfI HphI
MaeI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NspI PflMI
RsaI Sau3AI ScrFI StyI TfiI
Tru9I Tsp45I Tsp509I TspRI Van91I