##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MboII       MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI          578  AAATTC               [GA]^AATT[CT]
AfaI          302  GTAC                 GT^AC
AflII         432  CTTAAG               C^TTAAG
AlwI          103  GGATC                GGATC(4/5)
ApoI          578  AAATTC               [GA]^AATT[CT]
BciVI         218  GGATAC               GTATCC(6/5)
BfaI          385  CTAG                 C^TAG
BfrI          432  CTTAAG               C^TTAAG
BpmI          493  CTCCAG               CTGGAG(16/14)
BsaJI         516  CCTTGG               C^C[ACGT][ACGT]GG
Cfr13I        549  GGCCC                G^G[ACGT]CC
DdeI          455  CTAAG                C^T[ACGT]AG
DpnII         104  GATC                 ^GATC
DraII         548  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        311  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          320  GACTGACTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      548  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       516  CCTTGG               C^C[AT][AT]GG
HgaI          164  GACGC                GACGC(5/10)
HinfI          87  GAATC                G^A[ACGT]TC
HphI          280  GGTGA                GGTGA(8/7)
MaeI          385  CTAG                 C^TAG
MboI          104  GATC                 ^GATC
MfeI           38  CAATTG               C^AATTG
MnlI          141  CCTC                 CCTC(7/6)
MunI           38  CAATTG               C^AATTG
MwoI          187  GCTCGCATAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         104  GATC                 ^GATC
RsaI          302  GTAC                 GT^AC
Sau3AI        104  GATC                 ^GATC
Sau96I        549  GGCCC                G^G[ACGT]CC
SfaNI         167  GCATC                GCATC(5/9)
SmlI          432  CTTAAG               C^T[CT][GA]AG
StyI          516  CCTTGG               C^C[AT][AT]GG
TaqI          162  TCGA                 T^CGA
TfiI           87  GAATC                G^A[AT]TC
Tsp45I        178  GTGAC                ^GT[GC]AC
TthHB8I       162  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsrI          325  ACTGG                ACTGG(1/-1)
              440  ACTGG               
BssKI         392  CCAGG                ^CC[ACGT]GG
              568  CCTGG               
BstNI         392  CCAGG                CC^[AT]GG
              568  CCTGG               
Cac8I         187  GCTCGC               GC[ACGT]^[ACGT]GC
              199  GCTGGC              
EcoRII        392  CCAGG                ^CC[AT]GG
              568  CCTGG               
HaeIII        139  GGCC                 GG^CC
              549  GGCC                
MvaI          392  CCAGG                CC^[AT]GG
              568  CCTGG               
ScrFI         392  CCAGG                CC^[ACGT]GG
              568  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BbvI        BsiYI       BslI        Fnu4HI      
ItaI        MaeIII      MseI        Tru9I       TseI        
Tsp509I