Restriction map for
pF1KE0003
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI StuI StyI SwaI
Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 627 GACGTC GACGT^C
AccI 1429 GTCTAC GT^[AC][GT]AC
AciI 91 GCGG CCGC(-3/-1)
AcyI 627 GACGTC G[GA]^CG[CT]C
AflIII 1133 ACATGT A^C[GA][CT]GT
AlwNI 1496 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 861 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1464 GAGCTC G[AT]GC[AT]^C
AspI 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 1464 GAGCTC G[GA]GC[CT]^C
BbvI 466 GCTGC GCAGC(8/12)
BclI 1503 TGATCA T^GATCA
BmyI 1464 GAGCTC G[AGT]GC[ACT]^C
BpmI 189 CTGGAG CTGGAG(16/14)
BsaBI 802 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 627 GACGTC G[GA]^CG[CT]C
BsaI 38 GGTCTC GGTCTC(1/5)
BseRI 179 GAGGAG GAGGAG(10/8)
BsiHKAI 1464 GAGCTC G[AT]GC[AT]^C
BsmI 718 GCATTC GAATGC(1/-1)
Bsp1286I 1464 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 1133 ACATGT A^CATGT
BspMI 1148 GCAGGT ACCTGC(4/8)
BstAPI 718 GCATTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 942 GGATCT [GA]^GATC[CT]
Cac8I 244 GCTGGC GC[ACGT]^[ACGT]GC
DdeI 1035 CTCAG C^T[ACGT]AG
DraI 1044 TTTAAA TTT^AAA
EarI 355 CTCTTC CTCTTC(1/4)
EcoRI 775 GAATTC G^AATTC
FbaI 1503 TGATCA T^GATCA
Fnu4HI 466 GCTGC GC^[ACGT]GC
HapII 1006 CCGG C^CGG
Hin1I 627 GACGTC G[GA]^CG[CT]C
HpaII 1006 CCGG C^CGG
HphI 63 GGTGA GGTGA(8/7)
ItaI 466 GCTGC GC^[ACGT]GC
Ksp632I 355 CTCTTC CTCTTC(1/4)
MaeII 628 ACGT A^CGT
MamI 802 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 942 GGATCT [GA]^GATC[CT]
MspA1I 666 CAGCTG C[AC]G^C[GT]G
MspI 1006 CCGG C^CGG
NdeI 1118 CATATG CA^TATG
PaeR7I 1346 CTCGAG C^TCGAG
PflFI 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1033 GACTC GAGTC(4/5)
PstI 467 CTGCAG CTGCA^G
PvuII 666 CAGCTG CAG^CTG
SacI 1464 GAGCTC GAGCT^C
SfcI 467 CTGCAG C^T[GA][CT]AG
SmaI 1005 CCCGGG CCC^GGG
SpeI 1486 ACTAGT A^CTAGT
TseI 466 GCTGC G^C[AT]GC
Tth111I 490 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1346 CTCGAG C^TCGAG
XhoII 942 GGATCT [GA]^GATC[CT]
XmaCI 1005 CCCGGG C^CCGGG
XmaI 1005 CCCGGG C^CCGGG
XmnI 861 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 250 AAATTT [GA]^AATT[CT]
775 GAATTC
AfaI 640 GTAC GT^AC
1529 GTAC
ApoI 250 AAATTT [GA]^AATT[CT]
775 GAATTC
AvaI 1005 CCCGGG C^[CT]CG[GA]G
1346 CTCGAG
BbsI 1372 GAAGAC GAAGAC(2/6)
1437 GTCTTC
BcnI 1005 CCCGG CC^[GC]GG
1006 CCGGG
BfaI 176 CTAG C^TAG
1487 CTAG
BpuAI 1372 GAAGAC GAAGAC(2/6)
1437 GTCTTC
BsmAI 1130 GAGAC GTCTC(1/5)
39 GTCTC
BsoBI 1005 CCCGGG C^[CT]CG[GA]G
1346 CTCGAG
BspHI 455 TCATGA T^CATGA
981 TCATGA
DraII 95 AGGACCC [GA]G^G[ACGT]CC[CT]
475 GGGACCT
EcoO109I 95 AGGACCC [GA]G^G[ACGT]CC[CT]
475 GGGACCT
HaeIII 1003 GGCC GG^CC
1185 GGCC
MaeI 176 CTAG C^TAG
1487 CTAG
NciI 1005 CCCGG CC^[GC]GG
1006 CCGGG
NspI 833 ACATGC [GA]CATG^[CT]
1133 ACATGT
PpuMI 95 AGGACCC [GA]G^G[AT]CC[CT]
475 GGGACCT
RcaI 455 TCATGA T^CATGA
981 TCATGA
RsaI 640 GTAC GT^AC
1529 GTAC
SmlI 1346 CTCGAG C^T[CT][GA]AG
1461 CTTGAG
SspI 84 AATATT AAT^ATT
966 AATATT
TaqI 303 TCGA T^CGA
1347 TCGA
Tsp45I 496 GTCAC ^GT[GC]AC
511 GTCAC
TthHB8I 303 TCGA T^CGA
1347 TCGA
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI AvaII BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DpnII EcoRII FokI
HinfI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I Tsp509I TspRI