##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AvrII       BalI        
BamHI       BanII       BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     FauI        
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvaI        
MvnI        NaeI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScrFI       SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          321  AAATTC               [GA]^AATT[CT]
AcyI            3  GGCGCC               G[GA]^CG[CT]C
AfaI          295  GTAC                 GT^AC
ApoI          321  AAATTC               [GA]^AATT[CT]
AviII          72  TGCGCA               TGC^GCA
BanI            3  GGCGCC               G^G[CT][GA]CC
BbeI            3  GGCGCC               GGCGC^C
BciVI         312  GGATAC               GTATCC(6/5)
BfaI           49  CTAG                 C^TAG
BsaHI           3  GGCGCC               G[GA]^CG[CT]C
BsaI           37  GGTCTC               GGTCTC(1/5)
BsiYI         303  CCGGCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          303  CCGGCCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          38  GTCTC                GTCTC(1/5)
DraI          199  TTTAAA               TTT^AAA
EcoT22I       338  ATGCAT               ATGCA^T
FspI           72  TGCGCA               TGC^GCA
HaeII           3  GGCGCC               [GA]GCGC^[CT]
HapII         303  CCGG                 C^CGG
HgaI          387  GCGTC                GACGC(5/10)
Hin1I           3  GGCGCC               G[GA]^CG[CT]C
HpaII         303  CCGG                 C^CGG
HphI          258  TCACC                GGTGA(8/7)
KasI            3  GGCGCC               G^GCGCC
MaeI           49  CTAG                 C^TAG
MaeII         247  ACGT                 A^CGT
MnlI          347  CCTC                 CCTC(7/6)
MspA1I         68  CCGCTG               C[AC]G^C[GT]G
MspI          303  CCGG                 C^CGG
NarI            3  GGCGCC               GG^CGCC
NlaIII        207  CATG                 CATG^
NlaIV           3  GGCGCC               GG[ACGT]^[ACGT]CC
NsiI          338  ATGCAT               ATGCA^T
RsaI          295  GTAC                 GT^AC
ScaI          294  AGTACT               AGT^ACT
SfoI            3  GGCGCC               GGC^GCC
Tsp509I       322  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           59  CCGC                 CCGC(-3/-1)
               68  CCGC                
BbvI           75  GCAGC                GCAGC(8/12)
               70  GCTGC               
BstF5I        263  CATCC                GGATG(2/0)
              210  GGATG               
CfoI            4  GCGC                 GCG^C
               73  GCGC                
FokI          263  CATCC                GGATG(9/13)
              210  GGATG               
HaeIII        153  GGCC                 GG^CC
              305  GGCC                
HhaI            4  GCGC                 GCG^C
               73  GCGC                
HinP1I          4  GCGC                 G^CGC
               73  GCGC                
MwoI           52  GCCCACGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               61  GCCTTTTCCGC         
TaqI          291  TCGA                 T^CGA
              377  TCGA                
TseI           70  GCTGC                G^C[AT]GC
               75  GCAGC               
TspRI         119  CACTG                CA[GC]TG[ACGT][ACGT]^
              383  CACTG               
TthHB8I       291  TCGA                 T^CGA
              377  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

Fnu4HI      HinfI       ItaI        MaeIII      MboII       
MseI        SfaNI       TfiI        Tru9I