Restriction map for
pF1KB7683
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccII AclI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaI BseRI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HpaI
HphI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MluNI
MscI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 229 GACGTC GACGT^C
AccI 916 GTATAC GT^[AC][GT]AC
AccIII 10 TCCGGA T^CCGGA
AciI 876 CCGC CCGC(-3/-1)
AcsI 105 GAATTT [GA]^AATT[CT]
AcyI 229 GACGTC G[GA]^CG[CT]C
Alw44I 682 GTGCAC G^TGCAC
AlwI 394 GATCC GGATC(4/5)
AlwNI 299 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 815 AGCGCT AGC^GCT
ApaI 186 GGGCCC GGGCC^C
ApaLI 682 GTGCAC G^TGCAC
ApoI 105 GAATTT [GA]^AATT[CT]
AspHI 682 GTGCAC G[AT]GC[AT]^C
AvaII 487 GGACC G^G[AT]CC
BbsI 937 GAAGAC GAAGAC(2/6)
BglII 211 AGATCT A^GATCT
BpuAI 937 GAAGAC GAAGAC(2/6)
BsaHI 229 GACGTC G[GA]^CG[CT]C
BsaWI 10 TCCGGA [AT]^CCGG[AT]
BseAI 10 TCCGGA T^CCGGA
BsiHKAI 682 GTGCAC G[AT]GC[AT]^C
BsiYI 190 CCTGGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 190 CCTGGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 31 CGTCTC CGTCTC(1/5)
BsmFI 652 GTCCC GGGAC(10/14)
Bsp1407I 405 TGTACA T^GTACA
BspEI 10 TCCGGA T^CCGGA
BsrDI 611 GCAATG GCAATG(2/0)
BsrGI 405 TGTACA T^GTACA
Bst1107I 916 GTATAC GTA^TAC
BstF5I 1014 GGATG GGATG(2/0)
BstYI 211 AGATCT [GA]^GATC[CT]
BstZ17I 916 GTATAC GTA^TAC
DraII 186 GGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 815 AGCGCT AGC^GCT
EcoNI 190 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 186 GGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 875 CCCGC CCCGC(4/6)
FokI 1014 GGATG GGATG(9/13)
HaeII 815 AGCGCT [GA]GCGC^[CT]
HapII 11 CCGG C^CGG
HgaI 1018 GACGC GACGC(5/10)
Hin1I 229 GACGTC G[GA]^CG[CT]C
HindIII 644 AAGCTT A^AGCTT
HpaII 11 CCGG C^CGG
MflI 211 AGATCT [GA]^GATC[CT]
MroI 10 TCCGGA T^CCGGA
MslI 325 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 11 CCGG C^CGG
NlaIII 325 CATG CATG^
PleI 1034 GACTC GAGTC(4/5)
SfcI 477 CTGTAG C^T[GA][CT]AG
SspBI 405 TGTACA T^GTACA
SspI 469 AATATT AAT^ATT
TspRI 446 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 656 CCAAACCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 211 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 406 GTAC GT^AC
603 GTAC
BanII 186 GGGCCC G[GA]GC[CT]^C
787 GGGCTC
BsmAI 546 GAGAC GTCTC(1/5)
32 GTCTC
CfoI 811 GCGC GCG^C
816 GCGC
DpnII 212 GATC ^GATC
394 GATC
EarI 866 CTCTTC CTCTTC(1/4)
1009 GAAGAG
HhaI 811 GCGC GCG^C
816 GCGC
HinP1I 811 GCGC G^CGC
816 GCGC
Ksp632I 866 CTCTTC CTCTTC(1/4)
1009 GAAGAG
MaeII 230 ACGT A^CGT
671 ACGT
MboI 212 GATC ^GATC
394 GATC
NdeII 212 GATC ^GATC
394 GATC
NlaIV 186 GGGCCC GG[ACGT]^[ACGT]CC
872 GGCCCC
RsaI 406 GTAC GT^AC
603 GTAC
Sau3AI 212 GATC ^GATC
394 GATC
SfaNI 285 GATGC GCATC(5/9)
295 GATGC
SmlI 120 CTTGAG C^T[CT][GA]AG
976 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BbvI BmyI BsaJI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI HaeIII HinfI ItaI
MboII MnlI MseI MvaI MwoI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I