Restriction map for
pF1KB7671
##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AccII 195 CGCG CG^CG
AcyI 964 GACGCC G[GA]^CG[CT]C
AflIII 545 ACATGT A^C[GA][CT]GT
ApaI 759 GGGCCC GGGCC^C
AvaI 112 CCCGAG C^[CT]CG[GA]G
BbsI 306 GAAGAC GAAGAC(2/6)
BpuAI 306 GAAGAC GAAGAC(2/6)
BsaHI 964 GACGCC G[GA]^CG[CT]C
BsmBI 174 GAGACG CGTCTC(1/5)
BsoBI 112 CCCGAG C^[CT]CG[GA]G
BspLU11I 545 ACATGT A^CATGT
BsrI 204 CCAGT ACTGG(1/-1)
BstUI 195 CGCG CG^CG
EcoNI 294 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 964 GACGCC G[GA]^CG[CT]C
MvnI 195 CGCG CG^CG
PpuMI 446 AGGACCT [GA]G^G[AT]CC[CT]
PstI 589 CTGCAG CTGCA^G
SphI 179 GCATGC GCATG^C
StuI 950 AGGCCT AGG^CCT
TaqI 836 TCGA T^CGA
TspRI 960 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 836 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 282 GTAC GT^AC
549 GTAC
Aor51HI 341 AGCGCT AGC^GCT
494 AGCGCT
AviII 503 TGCGCA TGC^GCA
573 TGCGCA
BanII 79 GGGCTC G[GA]GC[CT]^C
759 GGGCCC
BlpI 246 GCTCAGC GC^T[ACGT]AGC
357 GCTGAGC
Bpu1102I 246 GCTCAGC GC^T[ACGT]AGC
357 GCTGAGC
BsmAI 115 GAGAC GTCTC(1/5)
174 GAGAC
BstAPI 576 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
648 GCAGCACCTGC
CelII 246 GCTCAGC GC^T[ACGT]AGC
357 GCTGAGC
DpnII 153 GATC ^GATC
705 GATC
Eco47III 341 AGCGCT AGC^GCT
494 AGCGCT
EcoT14I 755 CCATGG C^C[AT][AT]GG
968 CCATGG
FspI 503 TGCGCA TGC^GCA
573 TGCGCA
HaeII 341 AGCGCT [GA]GCGC^[CT]
494 AGCGCT
HgaI 176 GACGC GACGC(5/10)
964 GACGC
MaeIII 751 GTCAC ^GT[ACGT]AC
962 GTGAC
MboI 153 GATC ^GATC
705 GATC
MboII 306 GAAGA GAAGA(8/7)
287 TCTTC
MslI 739 CACATCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
810 CACCATCGTG
NcoI 755 CCATGG C^CATGG
968 CCATGG
NdeII 153 GATC ^GATC
705 GATC
NspI 179 GCATGC [GA]CATG^[CT]
545 ACATGT
PleI 117 GACTC GAGTC(4/5)
943 GACTC
RsaI 282 GTAC GT^AC
549 GTAC
Sau3AI 153 GATC ^GATC
705 GATC
SfaNI 510 GATGC GCATC(5/9)
188 GCATC
SfcI 84 CTGTAG C^T[GA][CT]AG
589 CTGCAG
StyI 755 CCATGG C^C[AT][AT]GG
968 CCATGG
TfiI 141 GATTC G^A[AT]TC
200 GATTC
Tsp45I 751 GTCAC ^GT[GC]AC
962 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BsrBI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PvuII Sau96I
ScrFI SmlI TseI