Restriction map for
pF1KB7737
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaBI BseAI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I TthHB8I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 514 GTCTAC GT^[AC][GT]AC
AcyI 847 GACGCC G[GA]^CG[CT]C
Alw44I 545 GTGCAC G^TGCAC
ApaLI 545 GTGCAC G^TGCAC
Asp700I 303 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 545 GTGCAC G[AT]GC[AT]^C
AspI 508 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1036 GCTGAGG CCTCAGC(-5/-2)
BcgI 528 CGACTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 332 TGATCA T^GATCA
BglI 889 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1133 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1133 GCTCAGC GC^T[ACGT]AGC
BsaHI 847 GACGCC G[GA]^CG[CT]C
BsaI 727 GAGACC GGTCTC(1/5)
BsaWI 432 TCCGGT [AT]^CCGG[AT]
BsiHKAI 545 GTGCAC G[AT]GC[AT]^C
BsmBI 845 GAGACG CGTCTC(1/5)
BsrBI 906 GAGCGG CCGCTC(-3/-3)
BssSI 11 CACGAG CACGAG(-5/-1)
BstAPI 630 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 966 AGATCC [GA]^GATC[CT]
CelII 1133 GCTCAGC GC^T[ACGT]AGC
DraIII 917 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 858 GAAGAG CTCTTC(1/4)
EcoNI 93 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 332 TGATCA T^GATCA
HgaI 847 GACGC GACGC(5/10)
Hin1I 847 GACGCC G[GA]^CG[CT]C
Ksp632I 858 GAAGAG CTCTTC(1/4)
MflI 966 AGATCC [GA]^GATC[CT]
NspI 356 GCATGT [GA]CATG^[CT]
PflFI 508 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 684 CTGCAG CTGCA^G
SexAI 918 ACCAGGT A^CC[AT]GGT
SfaNI 555 GATGC GCATC(5/9)
SfcI 684 CTGCAG C^T[GA][CT]AG
SgrAI 1205 CACCGGCG C[GA]^CCGG[CT]G
Tsp509I 17 AATT ^AATT
Tth111I 508 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 593 CCAACAGCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 966 AGATCC [GA]^GATC[CT]
XmnI 303 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 1190 GGTACC G^GTACC
1212 GGTACC
AccII 183 CGCG CG^CG
1266 CGCG
AlwI 624 GATCC GGATC(4/5)
967 GATCC
AlwNI 380 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
825 CAGAAACTG
Asp718I 1190 GGTACC G^GTACC
1212 GGTACC
BfaI 94 CTAG C^TAG
664 CTAG
BsgI 628 CTGCAC GTGCAG(16/14)
1051 CTGCAC
BsmFI 27 GTCCC GGGAC(10/14)
926 GTCCC
BstUI 183 CGCG CG^CG
1266 CGCG
EaeI 179 TGGCCG [CT]^GGCC[GA]
615 CGGCCA
HaeII 467 GGCGCT [GA]GCGC^[CT]
1130 GGCGCT
KpnI 1190 GGTACC GGTAC^C
1212 GGTACC
MaeI 94 CTAG C^TAG
664 CTAG
MslI 738 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1013 CACCTCTGTG
MvnI 183 CGCG CG^CG
1266 CGCG
NcoI 215 CCATGG C^CATGG
1068 CCATGG
SmaI 264 CCCGGG CCC^GGG
988 CCCGGG
StuI 896 AGGCCT AGG^CCT
1040 AGGCCT
TfiI 429 GATTC G^A[AT]TC
961 GAATC
XmaCI 264 CCCGGG C^CCGGG
988 CCCGGG
XmaI 264 CCCGGG C^CCGGG
988 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI StyI
TseI Tsp45I TspRI