Restriction map for
pF1KB5030
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanII
BbeI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FbaI
FokI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MaeI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 193 GTCTAC GT^[AC][GT]AC
AccII 94 CGCG CG^CG
AcsI 478 GAATTC [GA]^AATT[CT]
AflIII 140 ACGTGT A^C[GA][CT]GT
ApoI 478 GAATTC [GA]^AATT[CT]
AvaI 33 CCCGGG C^[CT]CG[GA]G
BalI 629 TGGCCA TGG^CCA
BbrPI 532 CACGTG CAC^GTG
BbsI 589 GAAGAC GAAGAC(2/6)
BbvCI 718 GCTGAGG CCTCAGC(-5/-2)
BcgI 50 GCAAGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 265 CTGGAG CTGGAG(16/14)
BpuAI 589 GAAGAC GAAGAC(2/6)
BsaAI 532 CACGTG [CT]AC^GT[GA]
BsaBI 237 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 19 GAGACC GGTCTC(1/5)
BseRI 106 GAGGAG GAGGAG(10/8)
BsgI 535 GTGCAG GTGCAG(16/14)
BsmBI 774 CGTCTC CGTCTC(1/5)
BsmFI 602 GGGAC GGGAC(10/14)
BsoBI 33 CCCGGG C^[CT]CG[GA]G
BspMI 332 ACCTGC ACCTGC(4/8)
BsrBI 438 CCGCTC CCGCTC(-3/-3)
BsrI 705 CCAGT ACTGG(1/-1)
BstUI 94 CGCG CG^CG
BstYI 408 GGATCT [GA]^GATC[CT]
CfoI 212 GCGC GCG^C
DdeI 719 CTGAG C^T[ACGT]AG
DrdI 373 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 629 TGGCCA [CT]^GGCC[GA]
EarI 743 GAAGAG CTCTTC(1/4)
EcoRI 478 GAATTC G^AATTC
EcoT22I 595 ATGCAT ATGCA^T
HaeII 211 GGCGCT [GA]GCGC^[CT]
HhaI 212 GCGC GCG^C
HinP1I 212 GCGC G^CGC
Ksp632I 743 GAAGAG CTCTTC(1/4)
KspI 93 CCGCGG CCGC^GG
MamI 237 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 408 GGATCT [GA]^GATC[CT]
MluNI 629 TGGCCA TGG^CCA
MscI 629 TGGCCA TGG^CCA
MslI 384 CATCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 94 CGCG CG^CG
NcoI 66 CCATGG C^CATGG
NsiI 595 ATGCAT ATGCA^T
NspI 593 ACATGC [GA]CATG^[CT]
PmaCI 532 CACGTG CAC^GTG
PmlI 532 CACGTG CAC^GTG
PpuMI 602 GGGACCT [GA]G^G[AT]CC[CT]
PstI 181 CTGCAG CTGCA^G
SacII 93 CCGCGG CCGC^GG
ScaI 53 AGTACT AGT^ACT
SexAI 605 ACCTGGT A^CC[AT]GGT
SfaNI 597 GCATC GCATC(5/9)
SfcI 181 CTGCAG C^T[GA][CT]AG
SmaI 33 CCCGGG CCC^GGG
SmlI 580 CTCAAG C^T[CT][GA]AG
TfiI 635 GATTC G^A[AT]TC
Tsp509I 479 AATT ^AATT
XcmI 404 CCAAGGATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 408 GGATCT [GA]^GATC[CT]
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AfaI 54 GTAC GT^AC
270 GTAC
AlwI 146 GGATC GGATC(4/5)
408 GGATC
BanI 169 GGCACC G^G[CT][GA]CC
466 GGCACC
BstXI 404 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCAGATTCCTGG
DraII 602 GGGACCT [GA]G^G[ACGT]CC[CT]
722 AGGCCCT
EcoO109I 602 GGGACCT [GA]G^G[ACGT]CC[CT]
722 AGGCCCT
FauI 314 CCCGC CCCGC(4/6)
95 GCGGG
MwoI 75 GCGGCTGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
458 GCTACCGAGGC
PflMI 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
693 CCAGCTGGTGG
PleI 373 GACTC GAGTC(4/5)
562 GACTC
RsaI 54 GTAC GT^AC
270 GTAC
TspRI 685 CACTG CA[GC]TG[ACGT][ACGT]^
706 CAGTG
Van91I 690 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
693 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AspHI AvaII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cac8I Cfr13I DpnII DsaI EcoRII
EcoT14I Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI StyI
TaqI TseI Tsp45I TthHB8I