Restriction map for
pF1KB6445
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AlwI AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I ClaI CpoI
DraI DraIII DrdI EaeI EagI
EarI EclXI Eco47III Eco52I EcoO65I
EcoT22I FbaI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeIII MamI
MflI MluI MluNI MroI MscI
MseI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SbfI SfaNI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 414 GGCGTC G[GA]^CG[CT]C
AflIII 551 ACACGT A^C[GA][CT]GT
AluI 82 AGCT AG^CT
AocI 880 CCTGAGG CC^T[ACGT]AGG
ApaI 444 GGGCCC GGGCC^C
BbsI 499 GAAGAC GAAGAC(2/6)
BbvCI 810 CCTCAGC CCTCAGC(-5/-2)
BcgI 692 GCAAGTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 9 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 18 GCTCAGC GC^T[ACGT]AGC
BpmI 78 CTGGAG CTGGAG(16/14)
Bpu1102I 18 GCTCAGC GC^T[ACGT]AGC
BpuAI 499 GAAGAC GAAGAC(2/6)
BsaHI 414 GGCGTC G[GA]^CG[CT]C
BsgI 51 GTGCAG GTGCAG(16/14)
BsmFI 510 GTCCC GGGAC(10/14)
BsrBI 72 GAGCGG CCGCTC(-3/-3)
BsrDI 792 CATTGC GCAATG(2/0)
BsrFI 914 ACCGGC [GA]^CCGG[CT]
BsrI 278 ACTGG ACTGG(1/-1)
BstXI 821 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 880 CCTGAGG CC^T[ACGT]AGG
CelII 18 GCTCAGC GC^T[ACGT]AGC
Cfr10I 914 ACCGGC [GA]^CCGG[CT]
DpnII 137 GATC ^GATC
DsaI 843 CCGCGG C^C[GA][CT]GG
Eco81I 880 CCTGAGG CC^T[ACGT]AGG
EcoNI 92 CCTATACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 931 GATATC GAT^ATC
EcoT14I 896 CCAAGG C^C[AT][AT]GG
HgaI 415 GCGTC GACGC(5/10)
Hin1I 414 GGCGTC G[GA]^CG[CT]C
KspI 843 CCGCGG CCGC^GG
MaeII 553 ACGT A^CGT
MboI 137 GATC ^GATC
MfeI 533 CAATTG C^AATTG
MunI 533 CAATTG C^AATTG
NdeII 137 GATC ^GATC
PaeR7I 333 CTCGAG C^TCGAG
PleI 373 GACTC GAGTC(4/5)
PpuMI 142 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 588 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 679 CTGCAG CTGCA^G
SacI 81 GAGCTC GAGCT^C
SacII 843 CCGCGG CCGC^GG
Sau3AI 137 GATC ^GATC
ScaI 157 AGTACT AGT^ACT
SexAI 503 ACCTGGT A^CC[AT]GGT
SfcI 679 CTGCAG C^T[GA][CT]AG
SmlI 333 CTCGAG C^T[CT][GA]AG
StuI 180 AGGCCT AGG^CCT
StyI 896 CCAAGG C^C[AT][AT]GG
TfiI 214 GATTC G^A[AT]TC
XhoI 333 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 685 CGCG CG^CG
844 CGCG
AcsI 31 GAATTC [GA]^AATT[CT]
526 GAATTC
AhdI 502 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACGGACTGTC
ApoI 31 GAATTC [GA]^AATT[CT]
526 GAATTC
AspEI 502 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACGGACTGTC
BanI 282 GGCACC G^G[CT][GA]CC
856 GGCACC
BseRI 307 CTCCTC GAGGAG(10/8)
430 GAGGAG
BstUI 685 CGCG CG^CG
844 CGCG
CfoI 684 GCGC GCG^C
796 GCGC
DraII 142 AGGACCT [GA]G^G[ACGT]CC[CT]
806 AGGCCCT
Eam1105I 502 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACGGACTGTC
EcoO109I 142 AGGACCT [GA]G^G[ACGT]CC[CT]
806 AGGCCCT
EcoRI 31 GAATTC G^AATTC
526 GAATTC
FauI 447 CCCGC CCCGC(4/6)
390 GCGGG
HhaI 684 GCGC GCG^C
796 GCGC
HinP1I 684 GCGC G^CGC
796 GCGC
HinfI 214 GATTC G^A[ACGT]TC
373 GACTC
HphI 262 GGTGA GGTGA(8/7)
665 TCACC
MslI 552 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
822 CATCCAGGTG
MspA1I 258 CAGCGG C[AC]G^C[GT]G
843 CCGCGG
MvnI 685 CGCG CG^CG
844 CGCG
SmaI 313 CCCGGG CCC^GGG
357 CCCGGG
XmaCI 313 CCCGGG C^CCGGG
357 CCCGGG
XmaI 313 CCCGGG C^CCGGG
357 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccI AciI AfaI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI TaqI TseI Tsp509I
TspRI TthHB8I