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# NO CUT ENZYMES ( 214 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MroI MseI MslI MspA1I MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tru9I Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 86 CGCG CG^CG
AfaI 6 GTAC GT^AC
AlwI 227 GGATC GGATC(4/5)
Asp700I 184 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 12 TGGCCA TGG^CCA
BbvI 45 GCAGC GCAGC(8/12)
BsiYI 9 CCGTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 9 CCGTGGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 268 GTCTC GTCTC(1/5)
BsrI 219 ACTGG ACTGG(1/-1)
BssKI 15 CCAGG ^CC[ACGT]GG
BstNI 15 CCAGG CC^[AT]GG
BstUI 86 CGCG CG^CG
DsaI 9 CCGTGG C^C[GA][CT]GG
EaeI 12 TGGCCA [CT]^GGCC[GA]
EarI 184 GAAGAG CTCTTC(1/4)
EcoRII 15 CCAGG ^CC[AT]GG
Fnu4HI 45 GCAGC GC^[ACGT]GC
HaeIII 13 GGCC GG^CC
ItaI 45 GCAGC GC^[ACGT]GC
Ksp632I 184 GAAGAG CTCTTC(1/4)
MluNI 12 TGGCCA TGG^CCA
MnlI 57 CCTC CCTC(7/6)
MscI 12 TGGCCA TGG^CCA
MvaI 15 CCAGG CC^[AT]GG
MvnI 86 CGCG CG^CG
MwoI 102 GCTCTATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 231 CATG CATG^
RsaI 6 GTAC GT^AC
ScrFI 15 CCAGG CC^[ACGT]GG
SfaNI 178 GATGC GCATC(5/9)
SspI 247 AATATT AAT^ATT
TseI 45 GCAGC G^C[AT]GC
XmnI 184 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AciI 87 GCGG CCGC(-3/-1)
123 GCGG
BsaJI 9 CCGTGG C^C[ACGT][ACGT]GG
15 CCAGGG
BsgI 28 GTGCAG GTGCAG(16/14)
43 GTGCAG
DpnII 83 GATC ^GATC
228 GATC
HinfI 90 GATTC G^A[ACGT]TC
189 GATTC
MboI 83 GATC ^GATC
228 GATC
MboII 184 GAAGA GAAGA(8/7)
62 TCTTC
NdeII 83 GATC ^GATC
228 GATC
Sau3AI 83 GATC ^GATC
228 GATC
TfiI 90 GATTC G^A[AT]TC
189 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 0 enzymes) #
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