Restriction map for
pF1KB6775
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
HphI KasI Ksp632I KspI MaeI
MaeIII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI NarI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp45I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 228 GGTACC G^GTACC
AcsI 67 GAATTT [GA]^AATT[CT]
AfaI 229 GTAC GT^AC
AgeI 76 ACCGGT A^CCGGT
AluI 44 AGCT AG^CT
ApoI 67 GAATTT [GA]^AATT[CT]
Asp700I 168 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 228 GGTACC G^GTACC
AspI 310 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 228 GGTACC G^G[CT][GA]CC
BbvI 45 GCTGC GCAGC(8/12)
BpmI 199 CTGGAG CTGGAG(16/14)
BsaJI 156 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 76 ACCGGT [AT]^CCGG[AT]
BsmFI 308 GGGAC GGGAC(10/14)
BsrBI 337 CCGCTC CCGCTC(-3/-3)
BsrI 265 CCAGT ACTGG(1/-1)
BssKI 156 CCTGG ^CC[ACGT]GG
BstNI 156 CCTGG CC^[AT]GG
Cac8I 346 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 303 GCGC GCG^C
EcoNI 173 CCTTCGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 156 CCTGG ^CC[AT]GG
HhaI 303 GCGC GCG^C
HinP1I 303 GCGC G^CGC
HincII 268 GTCAAC GT[CT]^[GA]AC
HindII 268 GTCAAC GT[CT]^[GA]AC
HinfI 310 GACTC G^A[ACGT]TC
KpnI 228 GGTACC GGTAC^C
MaeII 206 ACGT A^CGT
MboII 252 GAAGA GAAGA(8/7)
MseI 71 TTAA T^TAA
MvaI 156 CCTGG CC^[AT]GG
NaeI 346 GCCGGC GCC^GGC
NgoMIV 346 GCCGGC G^CCGGC
NlaIII 192 CATG CATG^
PflFI 310 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 76 ACCGGT A^CCGGT
PleI 310 GACTC GAGTC(4/5)
PstI 46 CTGCAG CTGCA^G
RsaI 229 GTAC GT^AC
ScrFI 156 CCTGG CC^[ACGT]GG
SfcI 46 CTGCAG C^T[GA][CT]AG
StuI 170 AGGCCT AGG^CCT
TaqI 176 TCGA T^CGA
Tru9I 71 TTAA T^TAA
TseI 45 GCTGC G^C[AT]GC
Tsp509I 68 AATT ^AATT
TspRI 184 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 310 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 176 TCGA T^CGA
XmnI 168 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 163 CGCG CG^CG
304 CGCG
AspHI 91 GTGCTC G[AT]GC[AT]^C
124 GTGCTC
AvaII 79 GGTCC G^G[AT]CC
324 GGTCC
BmyI 91 GTGCTC G[AGT]GC[ACT]^C
124 GTGCTC
BsiHKAI 91 GTGCTC G[AT]GC[AT]^C
124 GTGCTC
Bsp1286I 91 GTGCTC G[AGT]GC[ACT]^C
124 GTGCTC
BsrFI 76 ACCGGT [GA]^CCGG[CT]
346 GCCGGC
BstUI 163 CGCG CG^CG
304 CGCG
Cfr10I 76 ACCGGT [GA]^CCGG[CT]
346 GCCGGC
DdeI 224 CTGAG C^T[ACGT]AG
312 CTCAG
FauI 336 CCCGC CCCGC(4/6)
305 GCGGG
HaeIII 160 GGCC GG^CC
171 GGCC
HapII 77 CCGG C^CGG
347 CCGG
HpaII 77 CCGG C^CGG
347 CCGG
MspI 77 CCGG C^CGG
347 CCGG
MvnI 163 CGCG CG^CG
304 CGCG
MwoI 45 GCTGCAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
330 GCGGAACCCGC
NlaIV 228 GGTACC GG[ACGT]^[ACGT]CC
332 GGAACC
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BseRI BsiYI BslI Cfr13I
Fnu4HI ItaI MnlI Sau96I