##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssHII      BssKI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       ItaI        KasI        KpnI        KspI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvaI        MvnI        NaeI        NarI        NciI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        TseI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AfaI          157  GTAC                 GT^AC
AocI          253  CCTAAGG              CC^T[ACGT]AGG
AvrII         349  CCTAGG               C^CTAGG
BciVI         228  GGATAC               GTATCC(6/5)
BfaI          350  CTAG                 C^TAG
BlnI          349  CCTAGG               C^CTAGG
Bsp1407I      156  TGTACA               T^GTACA
BspHI          33  TCATGA               T^CATGA
BsrGI         156  TGTACA               T^GTACA
BssSI           7  CTCGTG               CACGAG(-5/-1)
Bsu36I        253  CCTAAGG              CC^T[ACGT]AGG
DraII         345  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EaeI          324  TGGCCG               [CT]^GGCC[GA]
Eco81I        253  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      345  GGGTCCT              [GA]G^G[ACGT]CC[CT]
HaeIII        325  GGCC                 GG^CC
HinfI          30  GAGTC                G^A[ACGT]TC
HphI          258  GGTGA                GGTGA(8/7)
MaeI          350  CTAG                 C^TAG
MaeII         321  ACGT                 A^CGT
MaeIII         43  GTAAC                ^GT[ACGT]AC
MseI          119  TTAA                 T^TAA
MslI           87  CACAGGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        160  CAGCTG               C[AC]G^C[GT]G
MwoI          162  GCTGGAAACGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI           75  CCATGG               C^CATGG
NlaIV         345  GGGTCC               GG[ACGT]^[ACGT]CC
PleI           30  GAGTC                GAGTC(4/5)
PpuMI         345  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         160  CAGCTG               CAG^CTG
RcaI           33  TCATGA               T^CATGA
RsaI          157  GTAC                 GT^AC
SspBI         156  TGTACA               T^GTACA
TaqI          180  TCGA                 T^CGA
Tru9I         119  TTAA                 T^TAA
TthHB8I       180  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AvaII          72  GGTCC                G^G[AT]CC
              346  GGTCC               
BstF5I         96  GGATG                GGATG(2/0)
              243  GGATG               
Cfr13I         72  GGTCC                G^G[ACGT]CC
              346  GGTCC               
DsaI           75  CCATGG               C^C[GA][CT]GG
              327  CCGTGG              
EcoT14I        75  CCATGG               C^C[AT][AT]GG
              349  CCTAGG              
FokI           96  GGATG                GGATG(9/13)
              243  GGATG               
Sau96I         72  GGTCC                G^G[ACGT]CC
              346  GGTCC               
StyI           75  CCATGG               C^C[AT][AT]GG
              349  CCTAGG              
Tsp509I        26  AATT                 ^AATT
               53  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BsaJI       DdeI        EarI        Ksp632I     
MboII       MnlI        NlaIII