##################################
# NO CUT ENZYMES ( 197 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AciI 11 GCGG CCGC(-3/-1)
BamHI 219 GGATCC G^GATCC
BanI 54 GGCACC G^G[CT][GA]CC
BbvI 33 GCTGC GCAGC(8/12)
BmyI 264 GTGCCC G[AGT]GC[ACT]^C
BpmI 95 CTGGAG CTGGAG(16/14)
BsiYI 21 CCCCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 21 CCCCTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 264 GTGCCC G[AGT]GC[ACT]^C
BstAPI 206 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 321 GGATG GGATG(2/0)
BstYI 219 GGATCC [GA]^GATC[CT]
DpnII 220 GATC ^GATC
EcoT14I 58 CCTTGG C^C[AT][AT]GG
FokI 321 GGATG GGATG(9/13)
HincII 226 GTTGAC GT[CT]^[GA]AC
HindII 226 GTTGAC GT[CT]^[GA]AC
HinfI 98 GAGTC G^A[ACGT]TC
MboI 220 GATC ^GATC
MflI 219 GGATCC [GA]^GATC[CT]
MseI 296 TTAA T^TAA
MslI 354 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 9 CAGCGG C[AC]G^C[GT]G
NdeII 220 GATC ^GATC
NspI 359 GCATGT [GA]CATG^[CT]
PleI 98 GAGTC GAGTC(4/5)
Sau3AI 220 GATC ^GATC
StyI 58 CCTTGG C^C[AT][AT]GG
Tru9I 296 TTAA T^TAA
TseI 33 GCTGC G^C[AT]GC
Tsp509I 307 AATT ^AATT
XhoII 219 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 93 AGCT AG^CT
393 AGCT
AlwI 220 GATCC GGATC(4/5)
219 GGATC
BalI 279 TGGCCA TGG^CCA
324 TGGCCA
Cac8I 36 GCTTGC GC[ACGT]^[ACGT]GC
332 GCAAGC
EaeI 279 TGGCCA [CT]^GGCC[GA]
324 TGGCCA
EarI 16 CTCTTC CTCTTC(1/4)
180 GAAGAG
Fnu4HI 11 GCGGC GC^[ACGT]GC
33 GCTGC
ItaI 11 GCGGC GC^[ACGT]GC
33 GCTGC
Ksp632I 16 CTCTTC CTCTTC(1/4)
180 GAAGAG
MluNI 279 TGGCCA TGG^CCA
324 TGGCCA
MscI 279 TGGCCA TGG^CCA
324 TGGCCA
MwoI 55 GCACCTTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
206 GCATCAAATGC
NlaIII 354 CATG CATG^
360 CATG
NlaIV 54 GGCACC GG[ACGT]^[ACGT]CC
219 GGATCC
SfaNI 291 GATGC GCATC(5/9)
206 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BsaJI BsrI BssKI BstNI DdeI
EcoRII HaeIII MaeIII MboII MnlI
MvaI ScrFI Tsp45I TspRI