Restriction map for
pF1KE0373
##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI Alw44I AlwNI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BspMI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MluI MluNI MroI MscI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AgeI 805 ACCGGT A^CCGGT
AocI 1806 CCTAAGG CC^T[ACGT]AGG
Aor51HI 296 AGCGCT AGC^GCT
Asp700I 1043 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 554 GAGCAC G[AT]GC[AT]^C
BanII 160 GAGCCC G[GA]GC[CT]^C
BcgI 90 CGAAGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 513 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1241 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 605 GGTCTC GGTCTC(1/5)
BsiHKAI 554 GAGCAC G[AT]GC[AT]^C
Bsp1407I 652 TGTACA T^GTACA
BsrDI 569 CATTGC GCAATG(2/0)
BsrGI 652 TGTACA T^GTACA
Bsu36I 1806 CCTAAGG CC^T[ACGT]AGG
DraII 828 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 296 AGCGCT AGC^GCT
Eco81I 1806 CCTAAGG CC^T[ACGT]AGG
EcoO109I 828 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 126 GAATTC G^AATTC
EcoT14I 1841 CCTTGG C^C[AT][AT]GG
HgaI 73 GCGTC GACGC(5/10)
MamI 1241 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1509 CAATTG C^AATTG
MspA1I 1115 CAGCTG C[AC]G^C[GT]G
MunI 1509 CAATTG C^AATTG
NdeI 1996 CATATG CA^TATG
NruI 1522 TCGCGA TCG^CGA
PflMI 1438 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 805 ACCGGT A^CCGGT
PleI 1556 GAGTC GAGTC(4/5)
PshAI 1826 GACAACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1670 CTGCAG CTGCA^G
PvuII 1115 CAGCTG CAG^CTG
SapI 3 GAAGAGC GCTCTTC(1/4)
SexAI 1017 ACCAGGT A^CC[AT]GGT
SfcI 1670 CTGCAG C^T[GA][CT]AG
SspBI 652 TGTACA T^GTACA
StyI 1841 CCTTGG C^C[AT][AT]GG
Van91I 1438 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1043 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 923 CGCG CG^CG
1523 CGCG
AciI 63 CCGC CCGC(-3/-1)
922 CCGC
AflIII 1345 ACATGT A^C[GA][CT]GT
1921 ACATGT
BsaWI 224 TCCGGT [AT]^CCGG[AT]
805 ACCGGT
BseRI 731 CTCCTC GAGGAG(10/8)
250 GAGGAG
BsmI 1140 GAATGC GAATGC(1/-1)
2112 GAATGC
BspLU11I 1345 ACATGT A^CATGT
1921 ACATGT
BsrFI 805 ACCGGT [GA]^CCGG[CT]
1780 GCCGGT
BstUI 923 CGCG CG^CG
1523 CGCG
BstYI 965 GGATCT [GA]^GATC[CT]
2145 GGATCT
Cfr10I 805 ACCGGT [GA]^CCGG[CT]
1780 GCCGGT
EaeI 341 TGGCCG [CT]^GGCC[GA]
1778 TGGCCG
HaeII 7 AGCGCC [GA]GCGC^[CT]
296 AGCGCT
MflI 965 GGATCT [GA]^GATC[CT]
2145 GGATCT
MslI 1983 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2088 CACAGATGTG
MvnI 923 CGCG CG^CG
1523 CGCG
NspI 1345 ACATGT [GA]CATG^[CT]
1921 ACATGT
SspI 379 AATATT AAT^ATT
1562 AATATT
TaqI 89 TCGA T^CGA
1866 TCGA
TfiI 766 GATTC G^A[AT]TC
1456 GAATC
TthHB8I 89 TCGA T^CGA
1866 TCGA
XhoII 965 GGATCT [GA]^GATC[CT]
2145 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp45I Tsp509I
TspRI