Restriction map for
pF1KE0038
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII AccII AccIII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspMI BsrBI BssHII
BssKI BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRII EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KspI MluI
MluNI MroI MscI MvaI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1654 GGTACC G^GTACC
AccI 1870 GTCTAC GT^[AC][GT]AC
AocI 745 CCTAAGG CC^T[ACGT]AGG
Asp718I 1654 GGTACC G^GTACC
BanI 1654 GGTACC G^G[CT][GA]CC
BanII 1775 GGGCTC G[GA]GC[CT]^C
BbsI 520 GTCTTC GAAGAC(2/6)
BglII 720 AGATCT A^GATCT
BpmI 1534 CTCCAG CTGGAG(16/14)
BpuAI 520 GTCTTC GAAGAC(2/6)
BsaAI 700 TACGTG [CT]AC^GT[GA]
BsaI 1531 GGTCTC GGTCTC(1/5)
Bsp1407I 1588 TGTACA T^GTACA
BsrFI 1306 GCCGGC [GA]^CCGG[CT]
BsrGI 1588 TGTACA T^GTACA
BstAPI 1111 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 745 CCTAAGG CC^T[ACGT]AGG
Cac8I 1306 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 1306 GCCGGC [GA]^CCGG[CT]
DraI 1078 TTTAAA TTT^AAA
DraIII 125 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 186 CTCTTC CTCTTC(1/4)
Eco81I 745 CCTAAGG CC^T[ACGT]AGG
EcoRV 1248 GATATC GAT^ATC
HaeIII 1309 GGCC GG^CC
HapII 1307 CCGG C^CGG
HgaI 65 GACGC GACGC(5/10)
HincII 1671 GTTGAC GT[CT]^[GA]AC
HindII 1671 GTTGAC GT[CT]^[GA]AC
HindIII 213 AAGCTT A^AGCTT
HpaII 1307 CCGG C^CGG
KpnI 1654 GGTACC GGTAC^C
Ksp632I 186 CTCTTC CTCTTC(1/4)
MfeI 1389 CAATTG C^AATTG
MspA1I 1815 CAGCTG C[AC]G^C[GT]G
MspI 1307 CCGG C^CGG
MunI 1389 CAATTG C^AATTG
NaeI 1306 GCCGGC GCC^GGC
NgoMIV 1306 GCCGGC G^CCGGC
PacI 427 TTAATTAA TTAAT^TAA
PvuII 1815 CAGCTG CAG^CTG
SmlI 1473 CTCAAG C^T[CT][GA]AG
SspBI 1588 TGTACA T^GTACA
TaqI 1243 TCGA T^CGA
TthHB8I 1243 TCGA T^CGA
XcmI 560 CCATAGTACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AseI 385 ATTAAT AT^TAAT
430 ATTAAT
AsnI 385 ATTAAT AT^TAAT
430 ATTAAT
AspHI 726 GAGCAC G[AT]GC[AT]^C
1823 GAGCAC
BsaBI 910 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1463 GATGCAAATC
BsaJI 939 CCAAGG C^C[ACGT][ACGT]GG
1967 CCAAGG
BsiHKAI 726 GAGCAC G[AT]GC[AT]^C
1823 GAGCAC
BsmAI 302 GTCTC GTCTC(1/5)
1532 GTCTC
BsmI 636 GAATGC GAATGC(1/-1)
1567 GAATGC
EcoRI 1751 GAATTC G^AATTC
2014 GAATTC
EcoT14I 939 CCAAGG C^C[AT][AT]GG
1967 CCAAGG
MaeII 701 ACGT A^CGT
1073 ACGT
MamI 910 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1463 GATGCAAATC
NlaIV 1410 GGAACC GG[ACGT]^[ACGT]CC
1654 GGTACC
PleI 751 GACTC GAGTC(4/5)
34 GAGTC
PshBI 385 ATTAAT AT^TAAT
430 ATTAAT
SfcI 1509 CTGTAG C^T[GA][CT]AG
1886 CTATAG
StyI 939 CCAAGG C^C[AT][AT]GG
1967 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
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AcsI AfaI AflIII AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BmyI BsiYI BslI Bsp1286I BspLU11I
BsrDI BsrI BstF5I BstYI Cfr13I
DdeI DpnII Fnu4HI FokI HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MwoI NdeII NlaIII NspI PflMI
RsaI Sau3AI Sau96I SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII XmnI