Restriction map for
pF1KE0366
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp700I AspEI AspI BbvCI
BcgI BciVI BfrI BglI BsaBI
BsaWI BseAI BsgI BsiWI BsmI
BspDI BspEI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 527 GGTACC G^GTACC
AcsI 1705 GAATTT [GA]^AATT[CT]
Aor51HI 992 AGCGCT AGC^GCT
ApoI 1705 GAATTT [GA]^AATT[CT]
Asp718I 527 GGTACC G^GTACC
AvaI 567 CTCGGG C^[CT]CG[GA]G
AviII 607 TGCGCA TGC^GCA
AvrII 339 CCTAGG C^CTAGG
BalI 950 TGGCCA TGG^CCA
BamHI 41 GGATCC G^GATCC
BbeI 736 GGCGCC GGCGC^C
BbrPI 1596 CACGTG CAC^GTG
BclI 1628 TGATCA T^GATCA
BglII 1724 AGATCT A^GATCT
BlnI 339 CCTAGG C^CTAGG
BlpI 55 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
BsiEI 94 CGGCCG CG[GA][CT]^CG
BsmBI 535 GAGACG CGTCTC(1/5)
BsoBI 567 CTCGGG C^[CT]CG[GA]G
BspLU11I 626 ACATGT A^CATGT
BsrBI 460 CCGCTC CCGCTC(-3/-3)
BsrFI 1465 GCCGGC [GA]^CCGG[CT]
BssSI 10 CTCGTG CACGAG(-5/-1)
CelII 55 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1465 GCCGGC [GA]^CCGG[CT]
DrdI 1333 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 94 CGGCCG C^GGCCG
EclXI 94 CGGCCG C^GGCCG
Eco47III 992 AGCGCT AGC^GCT
Eco52I 94 CGGCCG C^GGCCG
EcoNI 1164 CCTCTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1628 TGATCA T^GATCA
FspI 607 TGCGCA TGC^GCA
KasI 736 GGCGCC G^GCGCC
KpnI 527 GGTACC GGTAC^C
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
NaeI 1465 GCCGGC GCC^GGC
NarI 736 GGCGCC GG^CGCC
NgoMIV 1465 GCCGGC G^CCGGC
PflMI 575 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1596 CACGTG CAC^GTG
PmlI 1596 CACGTG CAC^GTG
PstI 1327 CTGCAG CTGCA^G
PvuII 1129 CAGCTG CAG^CTG
SbfI 1326 CCTGCAGG CCTGCA^GG
ScaI 1436 AGTACT AGT^ACT
SfoI 736 GGCGCC GGC^GCC
Sse8387I 1326 CCTGCAGG CCTGCA^GG
Tsp509I 1706 AATT ^AATT
Van91I 575 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 961 GTCTAC GT^[AC][GT]AC
1516 GTCTAC
Alw44I 630 GTGCAC G^TGCAC
883 GTGCAC
ApaLI 630 GTGCAC G^TGCAC
883 GTGCAC
BanI 527 GGTACC G^G[CT][GA]CC
736 GGCGCC
BfaI 31 CTAG C^TAG
340 CTAG
BsaAI 1519 TACGTG [CT]AC^GT[GA]
1596 CACGTG
BsaI 64 GAGACC GGTCTC(1/5)
649 GAGACC
Bsp1407I 623 TGTACA T^GTACA
668 TGTACA
BspHI 209 TCATGA T^CATGA
1559 TCATGA
BsrGI 623 TGTACA T^GTACA
668 TGTACA
BstAPI 117 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
279 GCAGCGGCTGC
BstYI 41 GGATCC [GA]^GATC[CT]
1724 AGATCT
EcoT14I 339 CCTAGG C^C[AT][AT]GG
1361 CCAAGG
KspI 639 CCGCGG CCGC^GG
1115 CCGCGG
MaeI 31 CTAG C^TAG
340 CTAG
MflI 41 GGATCC [GA]^GATC[CT]
1724 AGATCT
MslI 936 CACATCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1221 CACCAAGATG
RcaI 209 TCATGA T^CATGA
1559 TCATGA
SacI 312 GAGCTC GAGCT^C
504 GAGCTC
SacII 639 CCGCGG CCGC^GG
1115 CCGCGG
SapI 1254 GCTCTTC GCTCTTC(1/4)
1269 GCTCTTC
SfcI 1327 CTGCAG C^T[GA][CT]AG
1677 CTACAG
SspBI 623 TGTACA T^GTACA
668 TGTACA
StyI 339 CCTAGG C^C[AT][AT]GG
1361 CCAAGG
XhoII 41 GGATCC [GA]^GATC[CT]
1724 AGATCT
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StuI TaqI TseI Tsp45I TspRI
TthHB8I