Restriction map for
pF1KE0349
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanII BbeI BbrPI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoRV EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MluI
MroI MspA1I MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmlI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflIII 650 ACATGT A^C[GA][CT]GT
AocI 1853 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1388 AGCGCT AGC^GCT
AspI 835 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1211 CCCGGG C^[CT]CG[GA]G
AvaII 855 GGTCC G^G[AT]CC
BbsI 1774 GAAGAC GAAGAC(2/6)
BbvCI 2004 CCTCAGC CCTCAGC(-5/-2)
BfaI 2062 CTAG C^TAG
BglI 31 GCCATAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 212 CTGGAG CTGGAG(16/14)
BpuAI 1774 GAAGAC GAAGAC(2/6)
BsaBI 1804 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 847 GTGCAG GTGCAG(16/14)
BsmI 1712 GCATTC GAATGC(1/-1)
BsoBI 1211 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1181 TGTACA T^GTACA
BspHI 572 TCATGA T^CATGA
BspLU11I 650 ACATGT A^CATGT
BspMI 1822 ACCTGC ACCTGC(4/8)
BsrGI 1181 TGTACA T^GTACA
BstBI 1622 TTCGAA TT^CGAA
BstEII 1818 GGTAACC G^GT[ACGT]ACC
BstPI 1818 GGTAACC G^GT[ACGT]ACC
Bsu36I 1853 CCTTAGG CC^T[ACGT]AGG
DraI 1990 TTTAAA TTT^AAA
Eco47III 1388 AGCGCT AGC^GCT
Eco81I 1853 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1818 GGTAACC G^GT[ACGT]ACC
EcoRI 247 GAATTC G^AATTC
EcoT14I 778 CCTTGG C^C[AT][AT]GG
HgaI 1885 GACGC GACGC(5/10)
HincII 1882 GTTGAC GT[CT]^[GA]AC
HindII 1882 GTTGAC GT[CT]^[GA]AC
MaeI 2062 CTAG C^TAG
MamI 1804 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 149 CAATTG C^AATTG
MunI 149 CAATTG C^AATTG
NspV 1622 TTCGAA TT^CGAA
PflFI 835 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1631 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 854 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 572 TCATGA T^CATGA
SexAI 1652 ACCTGGT A^CC[AT]GGT
SfuI 1622 TTCGAA TT^CGAA
SmaI 1211 CCCGGG CCC^GGG
SphI 1007 GCATGC GCATG^C
SspBI 1181 TGTACA T^GTACA
StuI 528 AGGCCT AGG^CCT
StyI 778 CCTTGG C^C[AT][AT]GG
Tth111I 835 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1631 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1211 CCCGGG C^CCGGG
XmaI 1211 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AlwI 208 GGATC GGATC(4/5)
423 GGATC
AlwNI 206 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
562 CAGGCTCTG
AspHI 187 GAGCAC G[AT]GC[AT]^C
1000 GAGCAC
BalI 354 TGGCCA TGG^CCA
1541 TGGCCA
BciVI 112 GGATAC GTATCC(6/5)
962 GGATAC
BclI 575 TGATCA T^GATCA
1259 TGATCA
BsaI 1738 GAGACC GGTCTC(1/5)
2024 GAGACC
BsaWI 305 TCCGGT [AT]^CCGG[AT]
523 ACCGGA
BsiHKAI 187 GAGCAC G[AT]GC[AT]^C
1000 GAGCAC
BsmFI 1566 GGGAC GGGAC(10/14)
943 GTCCC
BstYI 208 GGATCT [GA]^GATC[CT]
423 GGATCT
DraII 854 GGGTCCT [GA]G^G[ACGT]CC[CT]
1787 AGGGCCT
EaeI 354 TGGCCA [CT]^GGCC[GA]
1541 TGGCCA
EarI 2035 GAAGAG CTCTTC(1/4)
2065 GAAGAG
EcoO109I 854 GGGTCCT [GA]G^G[ACGT]CC[CT]
1787 AGGGCCT
FauI 124 GCGGG CCCGC(4/6)
623 GCGGG
FbaI 575 TGATCA T^GATCA
1259 TGATCA
HaeII 128 GGCGCT [GA]GCGC^[CT]
1388 AGCGCT
Ksp632I 2035 GAAGAG CTCTTC(1/4)
2065 GAAGAG
MflI 208 GGATCT [GA]^GATC[CT]
423 GGATCT
MluNI 354 TGGCCA TGG^CCA
1541 TGGCCA
MscI 354 TGGCCA TGG^CCA
1541 TGGCCA
MslI 495 CATTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1008 CATGCTGGTG
NspI 650 ACATGT [GA]CATG^[CT]
1007 GCATGC
PstI 559 CTGCAG CTGCA^G
1824 CTGCAG
SfcI 559 CTGCAG C^T[GA][CT]AG
1824 CTGCAG
TaqI 1175 TCGA T^CGA
1623 TCGA
TthHB8I 1175 TCGA T^CGA
1623 TCGA
XcmI 410 CCAAGCTCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
808 CCAGCTTCAGTATGG
XhoII 208 GGATCT [GA]^GATC[CT]
423 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I BanI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI