Restriction map for
pF1KE0370
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# NO CUT ENZYMES ( 127 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 974 AAATTC [GA]^AATT[CT]
ApoI 974 AAATTC [GA]^AATT[CT]
AvaI 922 CCCGGG C^[CT]CG[GA]G
BamHI 1139 GGATCC G^GATCC
BanI 261 GGCACC G^G[CT][GA]CC
BsaAI 472 TACGTG [CT]AC^GT[GA]
BsaI 244 GAGACC GGTCTC(1/5)
BsiEI 944 CGGCCG CG[GA][CT]^CG
BsmBI 1329 CGTCTC CGTCTC(1/5)
BsmI 20 GCATTC GAATGC(1/-1)
BsoBI 922 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1055 TGTACA T^GTACA
BsrDI 1475 GCAATG GCAATG(2/0)
BsrFI 511 ACCGGC [GA]^CCGG[CT]
BsrGI 1055 TGTACA T^GTACA
BstAPI 861 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1139 GGATCC [GA]^GATC[CT]
Cfr10I 511 ACCGGC [GA]^CCGG[CT]
EagI 944 CGGCCG C^GGCCG
EclXI 944 CGGCCG C^GGCCG
Eco52I 944 CGGCCG C^GGCCG
EcoT14I 695 CCATGG C^C[AT][AT]GG
HaeII 365 AGCGCC [GA]GCGC^[CT]
MflI 1139 GGATCC [GA]^GATC[CT]
NcoI 695 CCATGG C^CATGG
PvuII 289 CAGCTG CAG^CTG
SapI 825 GAAGAGC GCTCTTC(1/4)
ScaI 152 AGTACT AGT^ACT
SfcI 1418 CTGTAG C^T[GA][CT]AG
SmaI 922 CCCGGG CCC^GGG
SmlI 1255 CTTGAG C^T[CT][GA]AG
SspBI 1055 TGTACA T^GTACA
StyI 695 CCATGG C^C[AT][AT]GG
Tsp509I 975 AATT ^AATT
XhoII 1139 GGATCC [GA]^GATC[CT]
XmaCI 922 CCCGGG C^CCGGG
XmaI 922 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 178 GACGTC GACGT^C
739 GACGTC
AccII 1209 CGCG CG^CG
1371 CGCG
AcyI 178 GACGTC G[GA]^CG[CT]C
739 GACGTC
AlwNI 286 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
862 CAGTGCCTG
BcnI 922 CCCGG CC^[GC]GG
923 CCGGG
BglI 682 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1084 GCCAAAAAGGC
BlpI 1281 GCTCAGC GC^T[ACGT]AGC
1548 GCTGAGC
Bpu1102I 1281 GCTCAGC GC^T[ACGT]AGC
1548 GCTGAGC
BsaHI 178 GACGTC G[GA]^CG[CT]C
739 GACGTC
BseRI 750 CTCCTC GAGGAG(10/8)
1453 CTCCTC
BsgI 156 CTGCAC GTGCAG(16/14)
502 GTGCAG
BstUI 1209 CGCG CG^CG
1371 CGCG
CelII 1281 GCTCAGC GC^T[ACGT]AGC
1548 GCTGAGC
EaeI 944 CGGCCG [CT]^GGCC[GA]
1394 CGGCCA
FauI 13 CCCGC CCCGC(4/6)
1093 GCGGG
HgaI 765 GACGC GACGC(5/10)
524 GCGTC
Hin1I 178 GACGTC G[GA]^CG[CT]C
739 GACGTC
MseI 134 TTAA T^TAA
1589 TTAA
MvnI 1209 CGCG CG^CG
1371 CGCG
NciI 922 CCCGG CC^[GC]GG
923 CCGGG
NspI 649 ACATGC [GA]CATG^[CT]
1487 ACATGC
PflMI 509 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
647 CCACATGCTGG
SacI 142 GAGCTC GAGCT^C
1099 GAGCTC
TaqI 6 TCGA T^CGA
114 TCGA
TfiI 467 GATTC G^A[AT]TC
1127 GAATC
Tru9I 134 TTAA T^TAA
1589 TTAA
TthHB8I 6 TCGA T^CGA
114 TCGA
Van91I 509 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
647 CCACATGCTGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI