Restriction map for
pF1KE0445
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI Hin1I HpaI KasI
KpnI KspI MaeII MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 733 GTCGAC GT^[AC][GT]AC
AlwNI 158 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 924 GGACC G^G[AT]CC
AviII 403 TGCGCA TGC^GCA
BbsI 946 GAAGAC GAAGAC(2/6)
BciVI 354 GTATCC GTATCC(6/5)
BclI 386 TGATCA T^GATCA
BcnI 825 CCGGG CC^[GC]GG
BfaI 325 CTAG C^TAG
BglI 11 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 522 AGATCT A^GATCT
BpuAI 946 GAAGAC GAAGAC(2/6)
BsaBI 384 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 770 GGTCTC GGTCTC(1/5)
BsmFI 827 GGGAC GGGAC(10/14)
BspHI 104 TCATGA T^CATGA
BsrDI 132 CATTGC GCAATG(2/0)
BstAPI 753 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 896 CCATTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 924 GGACC G^G[ACGT]CC
DraII 923 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 205 CTCTTC CTCTTC(1/4)
EcoO109I 923 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 386 TGATCA T^GATCA
FspI 403 TGCGCA TGC^GCA
HaeII 344 AGCGCC [GA]GCGC^[CT]
HapII 825 CCGG C^CGG
HgaI 491 GCGTC GACGC(5/10)
HindIII 873 AAGCTT A^AGCTT
HpaII 825 CCGG C^CGG
Ksp632I 205 CTCTTC CTCTTC(1/4)
MaeI 325 CTAG C^TAG
MamI 384 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 399 CAGCTG C[AC]G^C[GT]G
MspI 825 CCGG C^CGG
NciI 825 CCGGG CC^[GC]GG
PleI 141 GAGTC GAGTC(4/5)
PpuMI 923 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 399 CAGCTG CAG^CTG
RcaI 104 TCATGA T^CATGA
SalI 733 GTCGAC G^TCGAC
Sau96I 924 GGACC G^G[ACGT]CC
SfcI 812 CTACAG C^T[GA][CT]AG
SphI 510 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 243 GCGG CCGC(-3/-1)
412 GCGG
Alw44I 554 GTGCAC G^TGCAC
751 GTGCAC
AlwI 273 GATCC GGATC(4/5)
369 GATCC
ApaLI 554 GTGCAC G^TGCAC
751 GTGCAC
AspHI 554 GTGCAC G[AT]GC[AT]^C
751 GTGCAC
BalI 456 TGGCCA TGG^CCA
632 TGGCCA
BanI 260 GGCACC G^G[CT][GA]CC
280 GGTGCC
BpmI 911 CTCCAG CTGGAG(16/14)
518 CTGGAG
BsgI 603 CTGCAC GTGCAG(16/14)
760 CTGCAC
BsiHKAI 554 GTGCAC G[AT]GC[AT]^C
751 GTGCAC
BsmAI 203 GTCTC GTCTC(1/5)
771 GTCTC
CfoI 345 GCGC GCG^C
404 GCGC
EcoT14I 276 CCTTGG C^C[AT][AT]GG
453 CCTTGG
HhaI 345 GCGC GCG^C
404 GCGC
HinP1I 345 GCGC G^CGC
404 GCGC
HincII 733 GTCGAC GT[CT]^[GA]AC
916 GTTGAC
HindII 733 GTCGAC GT[CT]^[GA]AC
916 GTTGAC
HphI 312 GGTGA GGTGA(8/7)
893 TCACC
MboII 946 GAAGA GAAGA(8/7)
206 TCTTC
MluNI 456 TGGCCA TGG^CCA
632 TGGCCA
MscI 456 TGGCCA TGG^CCA
632 TGGCCA
NspI 337 GCATGT [GA]CATG^[CT]
510 GCATGC
StyI 276 CCTTGG C^C[AT][AT]GG
453 CCTTGG
TaqI 226 TCGA T^CGA
734 TCGA
TfiI 783 GAATC G^A[AT]TC
907 GAATC
TthHB8I 226 TCGA T^CGA
734 TCGA
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII