Restriction map for
pF1KE0374
##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BcgI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CpoI DraII
DraIII DrdI DsaI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HpaI KpnI
Ksp632I KspI MfeI MluI MroI
MunI NaeI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PvuI
RcaI RsrII SacII SalI SapI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 259 GACGTC GACGT^C
AccI 76 GTCTAC GT^[AC][GT]AC
AccII 68 CGCG CG^CG
AclI 1945 AACGTT AA^CGTT
AflIII 1453 ACGTGT A^C[GA][CT]GT
Aor51HI 1749 AGCGCT AGC^GCT
AseI 2271 ATTAAT AT^TAAT
AsnI 2271 ATTAAT AT^TAAT
Asp700I 475 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 40 CCCGAG C^[CT]CG[GA]G
BanI 113 GGCGCC G^G[CT][GA]CC
BanII 85 GAGCTC G[GA]GC[CT]^C
BbeI 113 GGCGCC GGCGC^C
BbvCI 1752 GCTGAGG CCTCAGC(-5/-2)
BciVI 487 GTATCC GTATCC(6/5)
BglII 1866 AGATCT A^GATCT
BsaAI 1452 TACGTG [CT]AC^GT[GA]
BsaBI 796 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 892 GTCCC GGGAC(10/14)
BsoBI 40 CCCGAG C^[CT]CG[GA]G
BspDI 1135 ATCGAT AT^CGAT
BspMI 17 GCAGGT ACCTGC(4/8)
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BsrFI 72 GCCGGT [GA]^CCGG[CT]
BssSI 106 CACGAG CACGAG(-5/-1)
BstUI 68 CGCG CG^CG
Cfr10I 72 GCCGGT [GA]^CCGG[CT]
ClaI 1135 ATCGAT AT^CGAT
DraI 1684 TTTAAA TTT^AAA
Eco47III 1749 AGCGCT AGC^GCT
HapII 73 CCGG C^CGG
HgaI 132 GACGC GACGC(5/10)
HincII 1518 GTTGAC GT[CT]^[GA]AC
HindII 1518 GTTGAC GT[CT]^[GA]AC
HindIII 1318 AAGCTT A^AGCTT
HpaII 73 CCGG C^CGG
KasI 113 GGCGCC G^GCGCC
MamI 796 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1065 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 73 CCGG C^CGG
MvnI 68 CGCG CG^CG
NarI 113 GGCGCC GG^CGCC
PflMI 687 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1521 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2271 ATTAAT AT^TAAT
Psp1406I 1945 AACGTT AA^CGTT
SacI 85 GAGCTC GAGCT^C
SbfI 117 CCTGCAGG CCTGCA^GG
ScaI 277 AGTACT AGT^ACT
SfoI 113 GGCGCC GGC^GCC
Sse8387I 117 CCTGCAGG CCTGCA^GG
SspI 787 AATATT AAT^ATT
Van91I 687 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 475 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1150 GAATTC [GA]^AATT[CT]
2044 GAATTC
AcyI 113 GGCGCC G[GA]^CG[CT]C
259 GACGTC
AlwNI 329 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
674 CAGCTGCTG
ApoI 1150 GAATTC [GA]^AATT[CT]
2044 GAATTC
AspHI 85 GAGCTC G[AT]GC[AT]^C
1242 GAGCAC
BsaHI 113 GGCGCC G[GA]^CG[CT]C
259 GACGTC
BsaI 346 GGTCTC GGTCTC(1/5)
696 GGTCTC
BsiEI 70 CGGCCG CG[GA][CT]^CG
1229 CGGCCG
BsiHKAI 85 GAGCTC G[AT]GC[AT]^C
1242 GAGCAC
BstXI 1064 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1466 CCATCATTCTGG
EagI 70 CGGCCG C^GGCCG
1229 CGGCCG
EclXI 70 CGGCCG C^GGCCG
1229 CGGCCG
Eco52I 70 CGGCCG C^GGCCG
1229 CGGCCG
EcoRI 1150 GAATTC G^AATTC
2044 GAATTC
EcoRV 1582 GATATC GAT^ATC
1882 GATATC
Hin1I 113 GGCGCC G[GA]^CG[CT]C
259 GACGTC
NdeI 311 CATATG CA^TATG
941 CATATG
SmlI 1625 CTCAAG C^T[CT][GA]AG
2204 CTCAAG
TaqI 1136 TCGA T^CGA
1676 TCGA
TthHB8I 1136 TCGA T^CGA
1676 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII