Restriction map for
pF1KB6366
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsgI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III EcoO109I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 391 GTCTAC GT^[AC][GT]AC
AccII 72 CGCG CG^CG
Alw44I 622 GTGCAC G^TGCAC
ApaLI 622 GTGCAC G^TGCAC
AspHI 622 GTGCAC G[AT]GC[AT]^C
BalI 224 TGGCCA TGG^CCA
BanII 514 GGGCTC G[GA]GC[CT]^C
BbeI 442 GGCGCC GGCGC^C
BclI 6 TGATCA T^GATCA
BcnI 41 CCGGG CC^[GC]GG
BfaI 410 CTAG C^TAG
BglI 364 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 488 AGATCT A^GATCT
BsaWI 355 TCCGGT [AT]^CCGG[AT]
BsiHKAI 622 GTGCAC G[AT]GC[AT]^C
BsmFI 757 GGGAC GGGAC(10/14)
Bsp1407I 749 TGTACA T^GTACA
BspMI 108 GCAGGT ACCTGC(4/8)
BsrGI 749 TGTACA T^GTACA
BstUI 72 CGCG CG^CG
BstYI 488 AGATCT [GA]^GATC[CT]
EagI 726 CGGCCG C^GGCCG
EarI 292 CTCTTC CTCTTC(1/4)
EclXI 726 CGGCCG C^GGCCG
Eco52I 726 CGGCCG C^GGCCG
EcoNI 206 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 396 CCCGC CCCGC(4/6)
FbaI 6 TGATCA T^GATCA
HincII 763 GTTGAC GT[CT]^[GA]AC
HindII 763 GTTGAC GT[CT]^[GA]AC
KasI 442 GGCGCC G^GCGCC
Ksp632I 292 CTCTTC CTCTTC(1/4)
KspI 71 CCGCGG CCGC^GG
MaeI 410 CTAG C^TAG
MflI 488 AGATCT [GA]^GATC[CT]
MluNI 224 TGGCCA TGG^CCA
MscI 224 TGGCCA TGG^CCA
MslI 627 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 72 CGCG CG^CG
NarI 442 GGCGCC GG^CGCC
NciI 41 CCGGG CC^[GC]GG
NcoI 462 CCATGG C^CATGG
NspI 117 GCATGC [GA]CATG^[CT]
PflMI 462 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 578 GAGTC GAGTC(4/5)
PstI 106 CTGCAG CTGCA^G
SacII 71 CCGCGG CCGC^GG
SapI 291 GCTCTTC GCTCTTC(1/4)
SexAI 415 ACCAGGT A^CC[AT]GGT
SfiI 363 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 442 GGCGCC GGC^GCC
SphI 117 GCATGC GCATG^C
SspBI 749 TGTACA T^GTACA
StuI 803 AGGCCT AGG^CCT
TaqI 86 TCGA T^CGA
TfiI 179 GAATC G^A[AT]TC
Tsp45I 658 GTGAC ^GT[GC]AC
TthHB8I 86 TCGA T^CGA
Van91I 462 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 488 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 184 CCTAAGG CC^T[ACGT]AGG
507 CCTGAGG
BanI 442 GGCGCC G^G[CT][GA]CC
817 GGTGCC
BmyI 514 GGGCTC G[AGT]GC[ACT]^C
622 GTGCAC
BpmI 210 CTGGAG CTGGAG(16/14)
771 CTGGAG
BsiEI 74 CGGTCG CG[GA][CT]^CG
726 CGGCCG
BsmAI 61 GTCTC GTCTC(1/5)
535 GTCTC
BsmBI 60 CGTCTC CGTCTC(1/5)
534 CGTCTC
Bsp1286I 514 GGGCTC G[AGT]GC[ACT]^C
622 GTGCAC
BstEII 217 GGTAACC G^GT[ACGT]ACC
343 GGTAACC
BstF5I 599 GGATG GGATG(2/0)
641 GGATG
BstPI 217 GGTAACC G^GT[ACGT]ACC
343 GGTAACC
Bsu36I 184 CCTAAGG CC^T[ACGT]AGG
507 CCTGAGG
Cac8I 117 GCATGC GC[ACGT]^[ACGT]GC
121 GCCAGC
CfoI 330 GCGC GCG^C
443 GCGC
DsaI 71 CCGCGG C^C[GA][CT]GG
462 CCATGG
Eco81I 184 CCTAAGG CC^T[ACGT]AGG
507 CCTGAGG
EcoO65I 217 GGTAACC G^GT[ACGT]ACC
343 GGTAACC
FokI 599 GGATG GGATG(9/13)
641 GGATG
HaeII 329 GGCGCT [GA]GCGC^[CT]
442 GGCGCC
HgaI 47 GCGTC GACGC(5/10)
352 GCGTC
HhaI 330 GCGC GCG^C
443 GCGC
HinP1I 330 GCGC G^CGC
443 GCGC
HinfI 179 GAATC G^A[ACGT]TC
578 GAGTC
HphI 4 GGTGA GGTGA(8/7)
174 GGTGA
PshAI 69 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
660 GACGGCAGTC
SfcI 106 CTGCAG C^T[GA][CT]AG
558 CTATAG
TspRI 135 CAGTG CA[GC]TG[ACGT][ACGT]^
696 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BbvI BsaHI BsaJI BsiYI
BslI BssKI BstNI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
HaeIII HapII Hin1I HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI