Restriction map for
pF1KE0450
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# NO CUT ENZYMES ( 117 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BcgI BciVI BcnI
BglII BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HindIII HinfI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 751 GACGTC GACGT^C
AflII 75 CTTAAG C^TTAAG
AflIII 1126 ACGTGT A^C[GA][CT]GT
AlwI 879 GATCC GGATC(4/5)
AocI 235 CCTGAGG CC^T[ACGT]AGG
ApaI 122 GGGCCC GGGCC^C
AspHI 994 GTGCTC G[AT]GC[AT]^C
AvaI 550 CTCGAG C^[CT]CG[GA]G
BbeI 297 GGCGCC GGCGC^C
BclI 929 TGATCA T^GATCA
BfaI 1120 CTAG C^TAG
BfrI 75 CTTAAG C^TTAAG
BpmI 264 CTCCAG CTGGAG(16/14)
BsaI 420 GGTCTC GGTCTC(1/5)
BsgI 1180 GTGCAG GTGCAG(16/14)
BsiHKAI 994 GTGCTC G[AT]GC[AT]^C
BsmBI 853 GAGACG CGTCTC(1/5)
BsoBI 550 CTCGAG C^[CT]CG[GA]G
BsrDI 900 CATTGC GCAATG(2/0)
BssSI 358 CTCGTG CACGAG(-5/-1)
BstEII 318 GGTCACC G^GT[ACGT]ACC
BstPI 318 GGTCACC G^GT[ACGT]ACC
BstYI 878 AGATCC [GA]^GATC[CT]
Bsu36I 235 CCTGAGG CC^T[ACGT]AGG
DraIII 166 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 235 CCTGAGG CC^T[ACGT]AGG
EcoO65I 318 GGTCACC G^GT[ACGT]ACC
FbaI 929 TGATCA T^GATCA
HapII 1056 CCGG C^CGG
HincII 517 GTCAAC GT[CT]^[GA]AC
HindII 517 GTCAAC GT[CT]^[GA]AC
HpaII 1056 CCGG C^CGG
KasI 297 GGCGCC G^GCGCC
MaeI 1120 CTAG C^TAG
MflI 878 AGATCC [GA]^GATC[CT]
MspI 1056 CCGG C^CGG
NarI 297 GGCGCC GG^CGCC
NcoI 115 CCATGG C^CATGG
NspI 80 GCATGC [GA]CATG^[CT]
PaeR7I 550 CTCGAG C^TCGAG
PvuII 958 CAGCTG CAG^CTG
SfiI 598 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 297 GGCGCC GGC^GCC
SphI 80 GCATGC GCATG^C
TaqI 551 TCGA T^CGA
TthHB8I 551 TCGA T^CGA
XcmI 1064 CCATCGTACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 550 CTCGAG C^TCGAG
XhoII 878 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 610 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
958 CAGCTGCTG
AvaII 70 GGTCC G^G[AT]CC
887 GGACC
BbrPI 862 CACGTG CAC^GTG
1125 CACGTG
BlpI 840 GCTCAGC GC^T[ACGT]AGC
1272 GCTGAGC
Bpu1102I 840 GCTCAGC GC^T[ACGT]AGC
1272 GCTGAGC
BsaAI 862 CACGTG [CT]AC^GT[GA]
1125 CACGTG
BseRI 997 CTCCTC GAGGAG(10/8)
394 GAGGAG
BsrBI 397 GAGCGG CCGCTC(-3/-3)
1275 GAGCGG
CelII 840 GCTCAGC GC^T[ACGT]AGC
1272 GCTGAGC
DsaI 115 CCATGG C^C[GA][CT]GG
917 CCGTGG
EarI 727 CTCTTC CTCTTC(1/4)
1012 CTCTTC
FauI 378 CCCGC CCCGC(4/6)
713 GCGGG
HgaI 364 GACGC GACGC(5/10)
855 GACGC
Ksp632I 727 CTCTTC CTCTTC(1/4)
1012 CTCTTC
MseI 7 TTAA T^TAA
76 TTAA
MspA1I 958 CAGCTG C[AC]G^C[GT]G
1150 CAGCGG
PflMI 175 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
609 CCAGTTTCTGG
PmaCI 862 CACGTG CAC^GTG
1125 CACGTG
PmlI 862 CACGTG CAC^GTG
1125 CACGTG
PpuMI 69 AGGTCCC [GA]G^G[AT]CC[CT]
886 GGGACCC
PstI 967 CTGCAG CTGCA^G
1210 CTGCAG
Tru9I 7 TTAA T^TAA
76 TTAA
Van91I 175 CCACCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
609 CCAGTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AfaI AluI BalI
BanI BanII BbsI BbvCI BbvI
BglI BmyI BpuAI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI Tsp45I TspRI