Restriction map for
pF1KB6393
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MflI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 658 GTAGAC GT^[AC][GT]AC
AfaI 423 GTAC GT^AC
AhdI 94 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 451 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 94 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 230 TGGCCA TGG^CCA
BanII 792 GAGCTC G[GA]GC[CT]^C
BbsI 588 GAAGAC GAAGAC(2/6)
BcgI 72 CGACTTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 326 TGATCA T^GATCA
BcnI 54 CCCGG CC^[GC]GG
BpuAI 588 GAAGAC GAAGAC(2/6)
BsaI 340 GAGACC GGTCTC(1/5)
BsmBI 101 CGTCTC CGTCTC(1/5)
BspHI 323 TCATGA T^CATGA
BspMI 381 GCAGGT ACCTGC(4/8)
BsrDI 696 CATTGC GCAATG(2/0)
BstF5I 213 CATCC GGATG(2/0)
CfoI 28 GCGC GCG^C
DrdI 304 GACACAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 243 CCATGG C^C[GA][CT]GG
EaeI 230 TGGCCA [CT]^GGCC[GA]
Eam1105I 94 GACTACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 243 CCATGG C^C[AT][AT]GG
FauI 648 CCCGC CCCGC(4/6)
FbaI 326 TGATCA T^GATCA
FokI 213 CATCC GGATG(9/13)
HapII 55 CCGG C^CGG
HgaI 582 GCGTC GACGC(5/10)
HhaI 28 GCGC GCG^C
HinP1I 28 GCGC G^CGC
HinfI 664 GAGTC G^A[ACGT]TC
HpaII 55 CCGG C^CGG
MluNI 230 TGGCCA TGG^CCA
MscI 230 TGGCCA TGG^CCA
MspA1I 295 CAGCTG C[AC]G^C[GT]G
MspI 55 CCGG C^CGG
NciI 54 CCCGG CC^[GC]GG
NcoI 243 CCATGG C^CATGG
PleI 664 GAGTC GAGTC(4/5)
PvuII 295 CAGCTG CAG^CTG
RcaI 323 TCATGA T^CATGA
RsaI 423 GTAC GT^AC
SacI 792 GAGCTC GAGCT^C
SexAI 464 ACCTGGT A^CC[AT]GGT
SfaNI 758 GCATC GCATC(5/9)
StyI 243 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AspHI 508 GTGCTC G[AT]GC[AT]^C
792 GAGCTC
AvaI 435 CCCGAG C^[CT]CG[GA]G
631 CCCGAG
AvaII 462 GGACC G^G[AT]CC
627 GGACC
BmyI 508 GTGCTC G[AGT]GC[ACT]^C
792 GAGCTC
BseRI 283 GAGGAG GAGGAG(10/8)
603 GAGGAG
BsiHKAI 508 GTGCTC G[AT]GC[AT]^C
792 GAGCTC
BsoBI 435 CCCGAG C^[CT]CG[GA]G
631 CCCGAG
Bsp1286I 508 GTGCTC G[AGT]GC[ACT]^C
792 GAGCTC
Cac8I 30 GCCTGC GC[ACGT]^[ACGT]GC
248 GCTGGC
DpnII 327 GATC ^GATC
742 GATC
MboI 327 GATC ^GATC
742 GATC
NdeII 327 GATC ^GATC
742 GATC
PflMI 413 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGTGCCTGG
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
626 AGGACCC
PstI 292 CTGCAG CTGCA^G
619 CTGCAG
Sau3AI 327 GATC ^GATC
742 GATC
SfcI 292 CTGCAG C^T[GA][CT]AG
619 CTGCAG
Tsp45I 92 GTGAC ^GT[GC]AC
574 GTCAC
Van91I 413 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
450 CCAGTGCCTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstNI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HphI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TaqI TseI TspRI TthHB8I