Restriction map for
pF1KB6704
##################################
# NO CUT ENZYMES ( 203 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HphI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MscI
MseI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 230 TCCGGA T^CCGGA
AlwI 358 GATCC GGATC(4/5)
BbvI 80 GCAGC GCAGC(8/12)
BciVI 297 GTATCC GTATCC(6/5)
BfaI 323 CTAG C^TAG
BsaJI 208 CCTGGG C^C[ACGT][ACGT]GG
BsaWI 230 TCCGGA [AT]^CCGG[AT]
BseAI 230 TCCGGA T^CCGGA
BsiYI 268 CCAGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 268 CCAGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 199 GAGAC GTCTC(1/5)
BspEI 230 TCCGGA T^CCGGA
BsrFI 43 ACCGGC [GA]^CCGG[CT]
BstF5I 75 GGATG GGATG(2/0)
BstXI 65 CCACTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 357 AGATCC [GA]^GATC[CT]
Cfr10I 43 ACCGGC [GA]^CCGG[CT]
Fnu4HI 80 GCAGC GC^[ACGT]GC
FokI 75 GGATG GGATG(9/13)
HgaI 4 GACGC GACGC(5/10)
ItaI 80 GCAGC GC^[ACGT]GC
MaeI 323 CTAG C^TAG
MaeIII 146 GTTAC ^GT[ACGT]AC
MflI 357 AGATCC [GA]^GATC[CT]
MroI 230 TCCGGA T^CCGGA
NlaIII 22 CATG CATG^
PstI 292 CTGCAG CTGCA^G
TseI 80 GCAGC G^C[AT]GC
XhoII 357 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 8 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
203 CAGCACCTG
BbvCI 317 CCTCAGC CCTCAGC(-5/-2)
90 GCTGAGG
BsmFI 95 GGGAC GGGAC(10/14)
168 GTCCC
BsrI 67 ACTGG ACTGG(1/-1)
301 CCAGT
BssKI 112 CCAGG ^CC[ACGT]GG
208 CCTGG
BstNI 112 CCAGG CC^[AT]GG
208 CCTGG
Cac8I 60 GCTTGC GC[ACGT]^[ACGT]GC
107 GCGTGC
DpnII 271 GATC ^GATC
358 GATC
EcoRII 112 CCAGG ^CC[AT]GG
208 CCTGG
HapII 44 CCGG C^CGG
231 CCGG
HpaII 44 CCGG C^CGG
231 CCGG
MboI 271 GATC ^GATC
358 GATC
MboII 309 GAAGA GAAGA(8/7)
355 GAAGA
MspI 44 CCGG C^CGG
231 CCGG
MvaI 112 CCAGG CC^[AT]GG
208 CCTGG
NdeII 271 GATC ^GATC
358 GATC
Sau3AI 271 GATC ^GATC
358 GATC
ScrFI 112 CCAGG CC^[ACGT]GG
208 CCTGG
SfcI 292 CTGCAG C^T[GA][CT]AG
341 CTACAG
TspRI 8 CAGTG CA[GC]TG[ACGT][ACGT]^
66 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AfaI AluI DdeI MnlI MwoI
RsaI