Restriction map for
pF1KB6524
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BstAPI BstBI Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 586 GTATAC GT^[AC][GT]AC
AlwNI 352 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 419 TGCGCA TGC^GCA
BamHI 800 GGATCC G^GATCC
BglI 216 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 965 GAGACC GGTCTC(1/5)
BsgI 1002 CTGCAC GTGCAG(16/14)
BsmBI 127 GAGACG CGTCTC(1/5)
BsrBI 1105 GAGCGG CCGCTC(-3/-3)
BsrDI 734 GCAATG GCAATG(2/0)
BsrFI 941 GCCGGC [GA]^CCGG[CT]
BsrI 728 ACTGG ACTGG(1/-1)
BssSI 31 CTCGTG CACGAG(-5/-1)
Bst1107I 586 GTATAC GTA^TAC
BstEII 873 GGTCACC G^GT[ACGT]ACC
BstPI 873 GGTCACC G^GT[ACGT]ACC
BstXI 249 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 800 GGATCC [GA]^GATC[CT]
BstZ17I 586 GTATAC GTA^TAC
Cfr10I 941 GCCGGC [GA]^CCGG[CT]
EcoO65I 873 GGTCACC G^GT[ACGT]ACC
FspI 419 TGCGCA TGC^GCA
HaeII 1129 AGCGCC [GA]GCGC^[CT]
HincII 724 GTTGAC GT[CT]^[GA]AC
HindII 724 GTTGAC GT[CT]^[GA]AC
MflI 800 GGATCC [GA]^GATC[CT]
MseI 593 TTAA T^TAA
NaeI 941 GCCGGC GCC^GGC
NgoMIV 941 GCCGGC G^CCGGC
NspI 150 GCATGT [GA]CATG^[CT]
PflMI 790 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 374 GGGACCC [GA]G^G[AT]CC[CT]
PstI 770 CTGCAG CTGCA^G
PvuII 511 CAGCTG CAG^CTG
SfcI 770 CTGCAG C^T[GA][CT]AG
Tru9I 593 TTAA T^TAA
Van91I 790 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 800 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 109 GTGCAC G^TGCAC
1093 GTGCAC
AlwI 801 GATCC GGATC(4/5)
800 GGATC
ApaLI 109 GTGCAC G^TGCAC
1093 GTGCAC
AspHI 109 GTGCAC G[AT]GC[AT]^C
1093 GTGCAC
BanII 295 GGGCTC G[GA]GC[CT]^C
496 GAGCCC
BsiHKAI 109 GTGCAC G[AT]GC[AT]^C
1093 GTGCAC
BspMI 212 ACCTGC ACCTGC(4/8)
456 ACCTGC
DpnII 289 GATC ^GATC
801 GATC
DsaI 540 CCGTGG C^C[GA][CT]GG
1011 CCACGG
EaeI 1043 CGGCCA [CT]^GGCC[GA]
1063 CGGCCA
EarI 13 CTCTTC CTCTTC(1/4)
960 GAAGAG
HinfI 21 GACTC G^A[ACGT]TC
488 GACTC
Ksp632I 13 CTCTTC CTCTTC(1/4)
960 GAAGAG
MaeII 130 ACGT A^CGT
521 ACGT
MboI 289 GATC ^GATC
801 GATC
NdeII 289 GATC ^GATC
801 GATC
PleI 21 GACTC GAGTC(4/5)
488 GACTC
Sau3AI 289 GATC ^GATC
801 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI