Restriction map for
pF1KB6381
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BsrI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AclI 402 AACGTT AA^CGTT
AlwNI 752 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 525 ATTAAT AT^TAAT
AsnI 525 ATTAAT AT^TAAT
BamHI 135 GGATCC G^GATCC
BanII 218 GGGCTC G[GA]GC[CT]^C
BbvI 536 GCAGC GCAGC(8/12)
BglII 739 AGATCT A^GATCT
BmyI 218 GGGCTC G[AGT]GC[ACT]^C
BpmI 749 CTCCAG CTGGAG(16/14)
BsaBI 94 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 782 CCCGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 782 CCCGATCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 474 GAATGC GAATGC(1/-1)
Bsp1286I 218 GGGCTC G[AGT]GC[ACT]^C
BspHI 380 TCATGA T^CATGA
BspMI 563 GCAGGT ACCTGC(4/8)
BsrFI 638 GCCGGT [GA]^CCGG[CT]
BssSI 227 CACGAG CACGAG(-5/-1)
BstAPI 148 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 44 CCAGG CC^[AT]GG
BstXI 717 CCAGAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 359 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 638 GCCGGT [GA]^CCGG[CT]
DraII 772 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 227 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 41 CGGCCA [CT]^GGCC[GA]
EcoO109I 772 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 44 CCAGG ^CC[AT]GG
EcoT14I 139 CCAAGG C^C[AT][AT]GG
HaeIII 42 GGCC GG^CC
HincII 802 GTTGAC GT[CT]^[GA]AC
HindII 802 GTTGAC GT[CT]^[GA]AC
HphI 463 GGTGA GGTGA(8/7)
MamI 94 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 44 CCAGG CC^[AT]GG
MwoI 148 GCAGATGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 210 ACATGC [GA]CATG^[CT]
PleI 353 GAGTC GAGTC(4/5)
PpuMI 772 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 525 ATTAAT AT^TAAT
Psp1406I 402 AACGTT AA^CGTT
PvuII 357 CAGCTG CAG^CTG
RcaI 380 TCATGA T^CATGA
SapI 711 GAAGAGC GCTCTTC(1/4)
SfcI 186 CTGTAG C^T[GA][CT]AG
SmlI 348 CTCAAG C^T[CT][GA]AG
StyI 139 CCAAGG C^C[AT][AT]GG
TfiI 65 GATTC G^A[AT]TC
TseI 536 GCAGC G^C[AT]GC
TspRI 807 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 40 GCGG CCGC(-3/-1)
409 GCGG
AcsI 127 GAATTT [GA]^AATT[CT]
339 GAATTT
ApoI 127 GAATTT [GA]^AATT[CT]
339 GAATTT
BcnI 575 CCCGG CC^[GC]GG
776 CCCGG
BfaI 215 CTAG C^TAG
593 CTAG
BsaJI 44 CCAGGG C^C[ACGT][ACGT]GG
139 CCAAGG
BstF5I 144 GGATG GGATG(2/0)
411 GGATG
DdeI 707 CTGAG C^T[ACGT]AG
743 CTAAG
EarI 236 GAAGAG CTCTTC(1/4)
711 GAAGAG
Fnu4HI 40 GCGGC GC^[ACGT]GC
536 GCAGC
FokI 144 GGATG GGATG(9/13)
411 GGATG
HinfI 65 GATTC G^A[ACGT]TC
353 GAGTC
ItaI 40 GCGGC GC^[ACGT]GC
536 GCAGC
Ksp632I 236 GAAGAG CTCTTC(1/4)
711 GAAGAG
MaeI 215 CTAG C^TAG
593 CTAG
MspA1I 38 CAGCGG C[AC]G^C[GT]G
357 CAGCTG
NciI 575 CCCGG CC^[GC]GG
776 CCCGG
NlaIII 211 CATG CATG^
381 CATG
TaqI 282 TCGA T^CGA
570 TCGA
Tsp45I 225 GTCAC ^GT[GC]AC
464 GTGAC
TthHB8I 282 TCGA T^CGA
570 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI AvaII BsaI BsmAI
BsmFI BssKI BstYI Cfr13I DpnII
HapII HpaII MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
NdeII NlaIV Sau3AI Sau96I ScrFI
SfaNI Tru9I Tsp509I XhoII