Restriction map for
pF1KB6354
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BsrI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeII MaeIII
MamI MluI MroI MseI NarI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 732 GTGCAC G^TGCAC
ApaI 9 GGGCCC GGGCC^C
ApaLI 732 GTGCAC G^TGCAC
BalI 84 TGGCCA TGG^CCA
BamHI 236 GGATCC G^GATCC
BcgI 455 GCATCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 806 TGATCA T^GATCA
BseRI 742 GAGGAG GAGGAG(10/8)
BsgI 355 GTGCAG GTGCAG(16/14)
BsiEI 426 CGACCG CG[GA][CT]^CG
BspHI 248 TCATGA T^CATGA
BsrFI 226 GCCGGC [GA]^CCGG[CT]
BssHII 64 GCGCGC G^CGCGC
BssSI 196 CACGAG CACGAG(-5/-1)
BstAPI 110 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 182 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 236 GGATCC [GA]^GATC[CT]
Cfr10I 226 GCCGGC [GA]^CCGG[CT]
DsaI 150 CCACGG C^C[GA][CT]GG
FbaI 806 TGATCA T^GATCA
HaeII 229 GGCGCT [GA]GCGC^[CT]
MfeI 114 CAATTG C^AATTG
MflI 236 GGATCC [GA]^GATC[CT]
MluNI 84 TGGCCA TGG^CCA
MscI 84 TGGCCA TGG^CCA
MslI 810 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 114 CAATTG C^AATTG
NaeI 226 GCCGGC GCC^GGC
NgoMIV 226 GCCGGC G^CCGGC
NheI 466 GCTAGC G^CTAGC
PpuMI 689 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 142 CAGCTG CAG^CTG
RcaI 248 TCATGA T^CATGA
SmlI 418 CTTGAG C^T[CT][GA]AG
Tsp509I 115 AATT ^AATT
XcmI 629 CCAAAATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 236 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 237 GATCC GGATC(4/5)
236 GGATC
AspHI 285 GTGCTC G[AT]GC[AT]^C
732 GTGCAC
AvaII 366 GGACC G^G[AT]CC
690 GGACC
BfaI 319 CTAG C^TAG
467 CTAG
BpmI 217 CTGGAG CTGGAG(16/14)
640 CTGGAG
BsiHKAI 285 GTGCTC G[AT]GC[AT]^C
732 GTGCAC
BsiYI 54 CCTGGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
227 CCGGCGCTTGG
BslI 54 CCTGGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
227 CCGGCGCTTGG
BsmFI 689 GGGAC GGGAC(10/14)
780 GGGAC
DpnII 237 GATC ^GATC
807 GATC
EaeI 84 TGGCCA [CT]^GGCC[GA]
131 CGGCCA
MaeI 319 CTAG C^TAG
467 CTAG
MboI 237 GATC ^GATC
807 GATC
NdeII 237 GATC ^GATC
807 GATC
NspI 170 ACATGC [GA]CATG^[CT]
413 ACATGC
Sau3AI 237 GATC ^GATC
807 GATC
TaqI 158 TCGA T^CGA
311 TCGA
TthHB8I 158 TCGA T^CGA
311 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BmyI BsaI BsaJI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DraII
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PstI
SapI Sau96I ScrFI SfaNI SfcI
TseI TspRI