Restriction map for
pF1KB6466
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BalI BamHI BbeI BbrPI
BbvCI BciVI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I TspRI Tth111I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 372 GGTACC G^GTACC
AflIII 812 ACATGT A^C[GA][CT]GT
AlwNI 826 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 372 GGTACC G^GTACC
AvrII 876 CCTAGG C^CTAGG
BanI 372 GGTACC G^G[CT][GA]CC
BbsI 881 GAAGAC GAAGAC(2/6)
BcgI 525 GCAACAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 887 TGATCA T^GATCA
BglII 158 AGATCT A^GATCT
BlnI 876 CCTAGG C^CTAGG
BpmI 570 CTCCAG CTGGAG(16/14)
BpuAI 881 GAAGAC GAAGAC(2/6)
BseRI 988 GAGGAG GAGGAG(10/8)
BsgI 925 CTGCAC GTGCAG(16/14)
BsmI 194 GAATGC GAATGC(1/-1)
BspDI 667 ATCGAT AT^CGAT
BspLU11I 812 ACATGT A^CATGT
BsrBI 97 GAGCGG CCGCTC(-3/-3)
BstYI 158 AGATCT [GA]^GATC[CT]
Cac8I 462 GCAAGC GC[ACGT]^[ACGT]GC
ClaI 667 ATCGAT AT^CGAT
EcoT14I 876 CCTAGG C^C[AT][AT]GG
FbaI 887 TGATCA T^GATCA
HaeII 319 AGCGCC [GA]GCGC^[CT]
KpnI 372 GGTACC GGTAC^C
MaeII 137 ACGT A^CGT
MflI 158 AGATCT [GA]^GATC[CT]
NspI 812 ACATGT [GA]CATG^[CT]
PstI 592 CTGCAG CTGCA^G
SapI 576 GAAGAGC GCTCTTC(1/4)
SfaNI 853 GATGC GCATC(5/9)
SmlI 512 CTCAAG C^T[CT][GA]AG
StyI 876 CCTAGG C^C[AT][AT]GG
Tsp45I 716 GTGAC ^GT[GC]AC
Tsp509I 623 AATT ^AATT
XhoII 158 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 352 GACGCC G[GA]^CG[CT]C
760 GACGCC
ApaI 81 GGGCCC GGGCC^C
105 GGGCCC
AspHI 567 GAGCTC G[AT]GC[AT]^C
991 GAGCTC
BsaHI 352 GACGCC G[GA]^CG[CT]C
760 GACGCC
BsiHKAI 567 GAGCTC G[AT]GC[AT]^C
991 GAGCTC
BsmAI 865 GAGAC GTCTC(1/5)
971 GAGAC
BstF5I 954 CATCC GGATG(2/0)
852 GGATG
CfoI 32 GCGC GCG^C
320 GCGC
DdeI 222 CTCAG C^T[ACGT]AG
832 CTGAG
FauI 29 CCCGC CCCGC(4/6)
119 CCCGC
FokI 954 CATCC GGATG(9/13)
852 GGATG
HgaI 352 GACGC GACGC(5/10)
760 GACGC
HhaI 32 GCGC GCG^C
320 GCGC
Hin1I 352 GACGCC G[GA]^CG[CT]C
760 GACGCC
HinP1I 32 GCGC G^CGC
320 GCGC
MaeIII 226 GTTAC ^GT[ACGT]AC
716 GTGAC
SacI 567 GAGCTC GAGCT^C
991 GAGCTC
SfcI 273 CTACAG C^T[GA][CT]AG
592 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstNI BstUI
Cfr13I DpnII DraII DsaI EarI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SmaI TaqI TfiI
TseI TthHB8I XmaCI XmaI