Restriction map for
pF1KB6336
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MfeI MflI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AclI 542 AACGTT AA^CGTT
AluI 601 AGCT AG^CT
AlwI 487 GATCC GGATC(4/5)
AlwNI 635 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 67 GGACC G^G[AT]CC
BalI 676 TGGCCA TGG^CCA
BbvI 582 GCAGC GCAGC(8/12)
BsaBI 424 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 69 ACCGGA [AT]^CCGG[AT]
BsgI 317 CTGCAC GTGCAG(16/14)
BsmFI 66 GGGAC GGGAC(10/14)
BspMI 634 GCAGGT ACCTGC(4/8)
BssKI 611 CCTGG ^CC[ACGT]GG
BstBI 495 TTCGAA TT^CGAA
BstNI 611 CCTGG CC^[AT]GG
BstXI 274 CCAAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 676 TGGCCA [CT]^GGCC[GA]
EcoRII 611 CCTGG ^CC[AT]GG
FauI 27 CCCGC CCCGC(4/6)
Fnu4HI 582 GCAGC GC^[ACGT]GC
HapII 70 CCGG C^CGG
HincII 298 GTCAAC GT[CT]^[GA]AC
HindII 298 GTCAAC GT[CT]^[GA]AC
HinfI 549 GAGTC G^A[ACGT]TC
HpaII 70 CCGG C^CGG
ItaI 582 GCAGC GC^[ACGT]GC
MaeII 543 ACGT A^CGT
MamI 424 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 676 TGGCCA TGG^CCA
MscI 676 TGGCCA TGG^CCA
MspI 70 CCGG C^CGG
MvaI 611 CCTGG CC^[AT]GG
NlaIII 586 CATG CATG^
NspV 495 TTCGAA TT^CGAA
PleI 549 GAGTC GAGTC(4/5)
Psp1406I 542 AACGTT AA^CGTT
PstI 89 CTGCAG CTGCA^G
ScaI 692 AGTACT AGT^ACT
ScrFI 611 CCTGG CC^[ACGT]GG
SexAI 610 ACCTGGT A^CC[AT]GGT
SfaNI 631 GATGC GCATC(5/9)
SfcI 89 CTGCAG C^T[GA][CT]AG
SfuI 495 TTCGAA TT^CGAA
TseI 582 GCAGC G^C[AT]GC
Tsp45I 373 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 28 CCGC CCGC(-3/-1)
228 CCGC
AfaI 151 GTAC GT^AC
693 GTAC
BsiYI 58 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
100 CCGAAGAAGGG
BslI 58 CCTGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
100 CCGAAGAAGGG
BsmAI 76 GAGAC GTCTC(1/5)
18 GTCTC
BsrI 15 CCAGT ACTGG(1/-1)
185 CCAGT
Cfr13I 3 GGCCC G^G[ACGT]CC
67 GGACC
DpnII 261 GATC ^GATC
487 GATC
HphI 484 GGTGA GGTGA(8/7)
808 GGTGA
MboI 261 GATC ^GATC
487 GATC
MseI 173 TTAA T^TAA
392 TTAA
NdeII 261 GATC ^GATC
487 GATC
RsaI 151 GTAC GT^AC
693 GTAC
Sau3AI 261 GATC ^GATC
487 GATC
Sau96I 3 GGCCC G^G[ACGT]CC
67 GGACC
TaqI 496 TCGA T^CGA
547 TCGA
Tru9I 173 TTAA T^TAA
392 TTAA
TthHB8I 496 TCGA T^CGA
547 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AcsI ApoI BseRI BstF5I Cac8I
EarI FokI HaeIII Ksp632I MaeIII
MboII MnlI MslI NlaIV Tsp509I
TspRI