Restriction map for
pF1KB8995
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 237 GTCTAC GT^[AC][GT]AC
AcyI 1176 GACGCC G[GA]^CG[CT]C
AflIII 1049 ACATGT A^C[GA][CT]GT
Alw44I 1008 GTGCAC G^TGCAC
AlwI 27 GATCC GGATC(4/5)
AlwNI 955 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1008 GTGCAC G^TGCAC
AspHI 1008 GTGCAC G[AT]GC[AT]^C
BbvCI 186 GCTGAGG CCTCAGC(-5/-2)
BfaI 145 CTAG C^TAG
BsaHI 1176 GACGCC G[GA]^CG[CT]C
BsaWI 116 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1008 GTGCAC G[AT]GC[AT]^C
BsmAI 120 GAGAC GTCTC(1/5)
BsmI 884 GCATTC GAATGC(1/-1)
BspLU11I 1049 ACATGT A^CATGT
BstAPI 954 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 982 GCGC GCG^C
CpoI 1025 CGGTCCG CG^G[AT]CCG
DpnII 27 GATC ^GATC
EcoNI 1149 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1037 CCCGC CCCGC(4/6)
HaeII 981 GGCGCT [GA]GCGC^[CT]
HgaI 1176 GACGC GACGC(5/10)
HhaI 982 GCGC GCG^C
Hin1I 1176 GACGCC G[GA]^CG[CT]C
HinP1I 982 GCGC G^CGC
HincII 913 GTCAAC GT[CT]^[GA]AC
HindII 913 GTCAAC GT[CT]^[GA]AC
MaeI 145 CTAG C^TAG
MboI 27 GATC ^GATC
MspA1I 55 CAGCTG C[AC]G^C[GT]G
NdeII 27 GATC ^GATC
NspI 1049 ACATGT [GA]CATG^[CT]
PleI 235 GAGTC GAGTC(4/5)
PpuMI 96 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 55 CAGCTG CAG^CTG
RsrII 1025 CGGTCCG CG^G[AT]CCG
Sau3AI 27 GATC ^GATC
StuI 561 AGGCCT AGG^CCT
TaqI 462 TCGA T^CGA
TfiI 343 GATTC G^A[AT]TC
Tsp45I 295 GTCAC ^GT[GC]AC
TthHB8I 462 TCGA T^CGA
XcmI 968 CCATCCTAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 141 GTAC GT^AC
1053 GTAC
AvaI 110 CCCGGG C^[CT]CG[GA]G
1167 CCCGGG
BpmI 176 CTCCAG CTGGAG(16/14)
565 CTGGAG
BseRI 13 CTCCTC GAGGAG(10/8)
209 CTCCTC
BsoBI 110 CCCGGG C^[CT]CG[GA]G
1167 CCCGGG
DdeI 187 CTGAG C^T[ACGT]AG
233 CTGAG
HinfI 235 GAGTC G^A[ACGT]TC
343 GATTC
HphI 1117 GGTGA GGTGA(8/7)
1214 TCACC
MaeIII 295 GTCAC ^GT[ACGT]AC
871 GTTAC
MboII 998 TCTTC GAAGA(8/7)
1001 TCTTC
RsaI 141 GTAC GT^AC
1053 GTAC
SfaNI 280 GATGC GCATC(5/9)
1091 GCATC
SmaI 110 CCCGGG CCC^GGG
1167 CCCGGG
Tsp509I 904 AATT ^AATT
1066 AATT
XmaCI 110 CCCGGG C^CCGGG
1167 CCCGGG
XmaI 110 CCCGGG C^CCGGG
1167 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI ApaI AvaII BalI
BanII BbvI BcnI BglI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII ItaI
MluNI MnlI MscI MslI MspI
MvaI MwoI NciI NcoI NlaIII
NlaIV Sau96I ScrFI StyI TseI
TspRI