Restriction map for
pF1KB8649
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
EaeI EagI Eam1105I EarI EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 346 GTCTAC GT^[AC][GT]AC
AccII 694 CGCG CG^CG
AcyI 913 GGCGTC G[GA]^CG[CT]C
AfaI 132 GTAC GT^AC
Aor51HI 857 AGCGCT AGC^GCT
BanII 416 GAGCCC G[GA]GC[CT]^C
BbsI 798 GAAGAC GAAGAC(2/6)
BcgI 266 GCAGGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 827 CTAG C^TAG
BpmI 904 CTCCAG CTGGAG(16/14)
BpuAI 798 GAAGAC GAAGAC(2/6)
BsaHI 913 GGCGTC G[GA]^CG[CT]C
BsmBI 1012 GAGACG CGTCTC(1/5)
BspHI 461 TCATGA T^CATGA
BsrI 506 ACTGG ACTGG(1/-1)
BssSI 678 CACGAG CACGAG(-5/-1)
BstUI 694 CGCG CG^CG
BstYI 722 AGATCC [GA]^GATC[CT]
DraII 148 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 871 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 43 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 857 AGCGCT AGC^GCT
EcoO109I 148 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 161 CCAAGG C^C[AT][AT]GG
EcoT22I 952 ATGCAT ATGCA^T
Hin1I 913 GGCGTC G[GA]^CG[CT]C
KspI 693 CCGCGG CCGC^GG
MaeI 827 CTAG C^TAG
MaeIII 52 GTCAC ^GT[ACGT]AC
MflI 722 AGATCC [GA]^GATC[CT]
MseI 403 TTAA T^TAA
MslI 235 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 694 CGCG CG^CG
NsiI 952 ATGCAT ATGCA^T
NspI 954 GCATGC [GA]CATG^[CT]
PpuMI 148 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 461 TCATGA T^CATGA
RsaI 132 GTAC GT^AC
SacII 693 CCGCGG CCGC^GG
SbfI 705 CCTGCAGG CCTGCA^GG
SmaI 732 CCCGGG CCC^GGG
SmlI 1027 CTCAAG C^T[CT][GA]AG
SphI 954 GCATGC GCATG^C
Sse8387I 705 CCTGCAGG CCTGCA^GG
StuI 1001 AGGCCT AGG^CCT
StyI 161 CCAAGG C^C[AT][AT]GG
TfiI 492 GATTC G^A[AT]TC
Tru9I 403 TTAA T^TAA
Tsp45I 52 GTCAC ^GT[GC]AC
TspRI 975 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 722 AGATCC [GA]^GATC[CT]
XmaCI 732 CCCGGG C^CCGGG
XmaI 732 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 23 TCCGGA T^CCGGA
200 TCCGGA
AciI 693 CCGC CCGC(-3/-1)
695 GCGG
AcsI 399 GAATTT [GA]^AATT[CT]
526 AAATTC
AlwI 198 GATCC GGATC(4/5)
723 GATCC
AlwNI 256 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
514 CAGGAGCTG
AocI 67 CCTGAGG CC^T[ACGT]AGG
880 CCTGAGG
ApoI 399 GAATTT [GA]^AATT[CT]
526 AAATTC
AspHI 673 GAGCAC G[AT]GC[AT]^C
742 GTGCTC
AvaI 90 CTCGGG C^[CT]CG[GA]G
732 CCCGGG
BanI 627 GGTGCC G^G[CT][GA]CC
876 GGTGCC
BcnI 732 CCCGG CC^[GC]GG
733 CCGGG
BlpI 309 GCTGAGC GC^T[ACGT]AGC
966 GCTCAGC
Bpu1102I 309 GCTGAGC GC^T[ACGT]AGC
966 GCTCAGC
BsaWI 23 TCCGGA [AT]^CCGG[AT]
200 TCCGGA
BseAI 23 TCCGGA T^CCGGA
200 TCCGGA
BsiHKAI 673 GAGCAC G[AT]GC[AT]^C
742 GTGCTC
BsoBI 90 CTCGGG C^[CT]CG[GA]G
732 CCCGGG
BspEI 23 TCCGGA T^CCGGA
200 TCCGGA
Bsu36I 67 CCTGAGG CC^T[ACGT]AGG
880 CCTGAGG
CelII 309 GCTGAGC GC^T[ACGT]AGC
966 GCTCAGC
Eco81I 67 CCTGAGG CC^T[ACGT]AGG
880 CCTGAGG
HaeII 282 GGCGCT [GA]GCGC^[CT]
857 AGCGCT
HgaI 1014 GACGC GACGC(5/10)
914 GCGTC
MroI 23 TCCGGA T^CCGGA
200 TCCGGA
NciI 732 CCCGG CC^[GC]GG
733 CCGGG
PleI 88 GACTC GAGTC(4/5)
1023 GAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AvaII BbvI BmyI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp509I TthHB8I