Restriction map for
pF1KB6425
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfaNI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 919 GAATTC [GA]^AATT[CT]
AfaI 825 GTAC GT^AC
AlwI 705 GGATC GGATC(4/5)
AlwNI 205 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 292 GGGCCC GGGCC^C
ApoI 919 GAATTC [GA]^AATT[CT]
BalI 477 TGGCCA TGG^CCA
BbvCI 574 GCTGAGG CCTCAGC(-5/-2)
BcgI 564 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 42 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 42 GCTGAGC GC^T[ACGT]AGC
BseRI 865 GAGGAG GAGGAG(10/8)
BsgI 143 GTGCAG GTGCAG(16/14)
BsmBI 227 CGTCTC CGTCTC(1/5)
BspMI 145 GCAGGT ACCTGC(4/8)
BsrDI 768 CATTGC GCAATG(2/0)
BssSI 781 CTCGTG CACGAG(-5/-1)
BstXI 239 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 42 GCTGAGC GC^T[ACGT]AGC
EcoRI 919 GAATTC G^AATTC
EcoRV 160 GATATC GAT^ATC
HinfI 118 GACTC G^A[ACGT]TC
MaeII 226 ACGT A^CGT
MaeIII 171 GTCAC ^GT[ACGT]AC
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
MspA1I 257 CAGCTG C[AC]G^C[GT]G
NspI 467 ACATGC [GA]CATG^[CT]
PleI 118 GACTC GAGTC(4/5)
PvuII 257 CAGCTG CAG^CTG
RsaI 825 GTAC GT^AC
SbfI 499 CCTGCAGG CCTGCA^GG
SfiI 714 GGCCACGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 347 CCCGGG CCC^GGG
Sse8387I 499 CCTGCAGG CCTGCA^GG
Tsp45I 171 GTCAC ^GT[GC]AC
TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 534 CCACACAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 347 CCCGGG C^[CT]CG[GA]G
645 CTCGGG
AvaII 505 GGACC G^G[AT]CC
550 GGTCC
BcnI 347 CCCGG CC^[GC]GG
348 CCGGG
BglI 424 GCCACCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
715 GCCACGGGGGC
BsaI 379 GAGACC GGTCTC(1/5)
487 GAGACC
BsmFI 464 GGGAC GGGAC(10/14)
696 GTCCC
BsoBI 347 CCCGGG C^[CT]CG[GA]G
645 CTCGGG
BssHII 6 GCGCGC G^CGCGC
32 GCGCGC
DpnII 97 GATC ^GATC
706 GATC
EaeI 477 TGGCCA [CT]^GGCC[GA]
668 TGGCCG
EcoT14I 296 CCAAGG C^C[AT][AT]GG
860 CCTTGG
FauI 442 CCCGC CCCGC(4/6)
887 GCGGG
HaeII 12 GGCGCT [GA]GCGC^[CT]
39 GGCGCT
MboI 97 GATC ^GATC
706 GATC
NaeI 54 GCCGGC GCC^GGC
279 GCCGGC
NciI 347 CCCGG CC^[GC]GG
348 CCGGG
NdeII 97 GATC ^GATC
706 GATC
NgoMIV 54 GCCGGC G^CCGGC
279 GCCGGC
PpuMI 504 AGGACCC [GA]G^G[AT]CC[CT]
549 AGGTCCT
PstI 49 CTGCAG CTGCA^G
500 CTGCAG
Sau3AI 97 GATC ^GATC
706 GATC
StuI 407 AGGCCT AGG^CCT
777 AGGCCT
StyI 296 CCAAGG C^C[AT][AT]GG
860 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MvaI
MvnI MwoI NlaIII NlaIV Sau96I
ScrFI SfcI TseI Tsp509I