Restriction map for
pF1KB7217
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 30 CCGC CCGC(-3/-1)
AspHI 305 GTGCTC G[AT]GC[AT]^C
BalI 897 TGGCCA TGG^CCA
BbsI 450 GAAGAC GAAGAC(2/6)
BbvI 728 GCTGC GCAGC(8/12)
BclI 384 TGATCA T^GATCA
BfaI 584 CTAG C^TAG
BmyI 305 GTGCTC G[AGT]GC[ACT]^C
BpuAI 450 GAAGAC GAAGAC(2/6)
BsaBI 491 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 158 GGTCTC GGTCTC(1/5)
BsaWI 155 TCCGGT [AT]^CCGG[AT]
BsiHKAI 305 GTGCTC G[AT]GC[AT]^C
BsmAI 159 GTCTC GTCTC(1/5)
BsmI 23 GCATTC GAATGC(1/-1)
Bsp1286I 305 GTGCTC G[AGT]GC[ACT]^C
BsrDI 128 CATTGC GCAATG(2/0)
BstBI 425 TTCGAA TT^CGAA
BstF5I 66 GGATG GGATG(2/0)
BstXI 439 CCAGAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 6 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 897 TGGCCA [CT]^GGCC[GA]
EcoO109I 6 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 378 GAATTC G^AATTC
FbaI 384 TGATCA T^GATCA
FokI 66 GGATG GGATG(9/13)
HapII 156 CCGG C^CGG
HpaII 156 CCGG C^CGG
HphI 935 TCACC GGTGA(8/7)
MaeI 584 CTAG C^TAG
MamI 491 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 897 TGGCCA TGG^CCA
MscI 897 TGGCCA TGG^CCA
MspA1I 30 CCGCTG C[AC]G^C[GT]G
MspI 156 CCGG C^CGG
NspV 425 TTCGAA TT^CGAA
PpuMI 6 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 775 CTGCAG CTGCA^G
SapI 307 GCTCTTC GCTCTTC(1/4)
SfuI 425 TTCGAA TT^CGAA
TseI 728 GCTGC G^C[AT]GC
XcmI 393 CCAGTACATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 378 GAATTC [GA]^AATT[CT]
924 GAATTT
AflIII 874 ACATGT A^C[GA][CT]GT
887 ACATGT
AluI 51 AGCT AG^CT
341 AGCT
ApoI 378 GAATTC [GA]^AATT[CT]
924 GAATTT
BsaJI 278 CCATGG C^C[ACGT][ACGT]GG
592 CCATGG
BseRI 161 CTCCTC GAGGAG(10/8)
164 CTCCTC
BsmFI 188 GGGAC GGGAC(10/14)
275 GTCCC
BspLU11I 874 ACATGT A^CATGT
887 ACATGT
BspMI 773 ACCTGC ACCTGC(4/8)
4 GCAGGT
BssKI 648 CCTGG ^CC[ACGT]GG
763 CCAGG
BstNI 648 CCTGG CC^[AT]GG
763 CCAGG
DpnII 151 GATC ^GATC
385 GATC
DsaI 278 CCATGG C^C[GA][CT]GG
592 CCATGG
EarI 308 CTCTTC CTCTTC(1/4)
250 GAAGAG
EcoRII 648 CCTGG ^CC[AT]GG
763 CCAGG
EcoT14I 278 CCATGG C^C[AT][AT]GG
592 CCATGG
Fnu4HI 29 GCCGC GC^[ACGT]GC
728 GCTGC
HaeIII 597 GGCC GG^CC
898 GGCC
ItaI 29 GCCGC GC^[ACGT]GC
728 GCTGC
Ksp632I 308 CTCTTC CTCTTC(1/4)
250 GAAGAG
MboI 151 GATC ^GATC
385 GATC
MseI 194 TTAA T^TAA
202 TTAA
MvaI 648 CCTGG CC^[AT]GG
763 CCAGG
MwoI 23 GCATTCGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
531 GCCTGACATGC
NcoI 278 CCATGG C^CATGG
592 CCATGG
NdeII 151 GATC ^GATC
385 GATC
Sau3AI 151 GATC ^GATC
385 GATC
ScrFI 648 CCTGG CC^[ACGT]GG
763 CCAGG
SfcI 696 CTGTAG C^T[GA][CT]AG
775 CTGCAG
StyI 278 CCATGG C^C[AT][AT]GG
592 CCATGG
TaqI 426 TCGA T^CGA
822 TCGA
Tru9I 194 TTAA T^TAA
202 TTAA
TthHB8I 426 TCGA T^CGA
822 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AfaI AvaII BsrI Cfr13I DdeI
HinfI MaeIII MboII MnlI MslI
NlaIII NlaIV NspI RsaI Sau96I
TfiI Tsp45I Tsp509I TspRI