##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI            5  GTAC                 GT^AC
AhdI           73  GACCAGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI           98  AGCT                 AG^CT
AlwI           67  GATCC                GGATC(4/5)
AocI           19  CCTGAGG              CC^T[ACGT]AGG
AspEI          73  GACCAGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         136  GGTCC                G^G[AT]CC
BbvI           52  GCAGC                GCAGC(8/12)
BfaI          148  CTAG                 C^TAG
BsmAI          15  GTCTC                GTCTC(1/5)
BstF5I         87  GGATG                GGATG(2/0)
Bsu36I         19  CCTGAGG              CC^T[ACGT]AGG
Cfr13I        136  GGTCC                G^G[ACGT]CC
DdeI           20  CTGAG                C^T[ACGT]AG
Eam1105I       73  GACCAGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           30  GAAGAG               CTCTTC(1/4)
Eco81I         19  CCTGAGG              CC^T[ACGT]AGG
EcoRV         228  GATATC               GAT^ATC
Fnu4HI         52  GCAGC                GC^[ACGT]GC
FokI           87  GGATG                GGATG(9/13)
HinfI         105  GATTC                G^A[ACGT]TC
HphI           64  GGTGA                GGTGA(8/7)
ItaI           52  GCAGC                GC^[ACGT]GC
Ksp632I        30  GAAGAG               CTCTTC(1/4)
MaeI          148  CTAG                 C^TAG
RsaI            5  GTAC                 GT^AC
Sau96I        136  GGTCC                G^G[ACGT]CC
ScaI            4  AGTACT               AGT^ACT
TfiI          105  GATTC                G^A[AT]TC
TseI           52  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AcsI          115  GAATTC               [GA]^AATT[CT]
              205  GAATTC              
ApoI          115  GAATTC               [GA]^AATT[CT]
              205  GAATTC              
DpnII          67  GATC                 ^GATC
              154  GATC                
EcoRI         115  GAATTC               G^AATTC
              205  GAATTC              
MboI           67  GATC                 ^GATC
              154  GATC                
MboII          30  GAAGA                GAAGA(8/7)
              202  GAAGA               
MnlI           22  GAGG                 CCTC(7/6)
               33  GAGG                
NdeII          67  GATC                 ^GATC
              154  GATC                
Sau3AI         67  GATC                 ^GATC
              154  GATC                
Tsp509I       116  AATT                 ^AATT
              206  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

BsrI