##################################
# NO CUT ENZYMES (  189 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI           54  GTAC                 GT^AC
AspHI         121  GAGCTC               G[AT]GC[AT]^C
BalI           38  TGGCCA               TGG^CCA
BanII         121  GAGCTC               G[GA]GC[CT]^C
BbsI          172  GAAGAC               GAAGAC(2/6)
BbvI          141  GCTGC                GCAGC(8/12)
BmyI          121  GAGCTC               G[AGT]GC[ACT]^C
BpuAI         172  GAAGAC               GAAGAC(2/6)
BsaWI         182  ACCGGA               [AT]^CCGG[AT]
BseRI          31  CTCCTC               GAGGAG(10/8)
BsiHKAI       121  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      121  GAGCTC               G[AGT]GC[ACT]^C
BssSI          34  CTCGTG               CACGAG(-5/-1)
BstF5I        147  GGATG                GGATG(2/0)
Cac8I           4  GCATGC               GC[ACGT]^[ACGT]GC
DdeI           85  CTGAG                C^T[ACGT]AG
DraII         232  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI           38  TGGCCA               [CT]^GGCC[GA]
EcoNI          12  CCTGGATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      232  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       236  CCTTGG               C^C[AT][AT]GG
Fnu4HI        141  GCTGC                GC^[ACGT]GC
FokI          147  GGATG                GGATG(9/13)
ItaI          141  GCTGC                GC^[ACGT]GC
MluNI          38  TGGCCA               TGG^CCA
MscI           38  TGGCCA               TGG^CCA
NlaIII          5  CATG                 CATG^
NlaIV         232  GGGGCC               GG[ACGT]^[ACGT]CC
NspI            4  GCATGC               [GA]CATG^[CT]
PstI          142  CTGCAG               CTGCA^G
RsaI           54  GTAC                 GT^AC
SacI          121  GAGCTC               GAGCT^C
ScaI           53  AGTACT               AGT^ACT
SfaNI         148  GATGC                GCATC(5/9)
SfcI          142  CTGCAG               C^T[GA][CT]AG
SmlI          262  CTCAAG               C^T[CT][GA]AG
SphI            4  GCATGC               GCATG^C
StyI          236  CCTTGG               C^C[AT][AT]GG
TaqI          136  TCGA                 T^CGA
TseI          141  GCTGC                G^C[AT]GC
Tsp509I       156  AATT                 ^AATT
TthHB8I       136  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AlwI          111  GATCC                GGATC(4/5)
               15  GGATC               
AvaII         180  GGACC                G^G[AT]CC
              192  GGACC               
HapII          59  CCGG                 C^CGG
              183  CCGG                
HpaII          59  CCGG                 C^CGG
              183  CCGG                
MaeIII         71  GTGAC                ^GT[ACGT]AC
              220  GTCAC               
MboII         172  GAAGA                GAAGA(8/7)
               44  TCTTC               
MspI           59  CCGG                 C^CGG
              183  CCGG                
MwoI           99  GCTGAAGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              151  GCTGAAATTGC         
Tsp45I         71  GTGAC                ^GT[GC]AC
              220  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BssKI       
BstNI       Cfr13I      DpnII       EcoRII      HaeIII      
HphI        MboI        MnlI        MvaI        NdeII       
Sau3AI      Sau96I      ScrFI