Restriction map for
pF1KE0367
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AviII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BglI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DsaI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AflII 430 CTTAAG C^TTAAG
AhdI 382 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 930 GGATC GGATC(4/5)
AlwNI 2110 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 677 AGCGCT AGC^GCT
Asp700I 736 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 382 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 179 CCCGAG C^[CT]CG[GA]G
AvaII 1230 GGACC G^G[AT]CC
AvrII 1782 CCTAGG C^CTAGG
BalI 1502 TGGCCA TGG^CCA
BciVI 330 GTATCC GTATCC(6/5)
BcnI 1989 CCGGG CC^[GC]GG
BfrI 430 CTTAAG C^TTAAG
BglII 1318 AGATCT A^GATCT
BlnI 1782 CCTAGG C^CTAGG
BmyI 758 GTGCCC G[AGT]GC[ACT]^C
BsaBI 649 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2193 CTCCTC GAGGAG(10/8)
BsoBI 179 CCCGAG C^[CT]CG[GA]G
Bsp1286I 758 GTGCCC G[AGT]GC[ACT]^C
BspMI 1285 ACCTGC ACCTGC(4/8)
CfoI 678 GCGC GCG^C
Cfr13I 1230 GGACC G^G[ACGT]CC
DraII 1229 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 417 GACATTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1502 TGGCCA [CT]^GGCC[GA]
Eam1105I 382 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 677 AGCGCT AGC^GCT
EcoO109I 1229 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1782 CCTAGG C^C[AT][AT]GG
FauI 1642 GCGGG CCCGC(4/6)
HaeII 677 AGCGCT [GA]GCGC^[CT]
HapII 1989 CCGG C^CGG
HhaI 678 GCGC GCG^C
HinP1I 678 GCGC G^CGC
HincII 2044 GTTAAC GT[CT]^[GA]AC
HindII 2044 GTTAAC GT[CT]^[GA]AC
HindIII 985 AAGCTT A^AGCTT
HpaI 2044 GTTAAC GTT^AAC
HpaII 1989 CCGG C^CGG
MaeII 826 ACGT A^CGT
MamI 649 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1502 TGGCCA TGG^CCA
MscI 1502 TGGCCA TGG^CCA
MspA1I 2110 CAGCTG C[AC]G^C[GT]G
MspI 1989 CCGG C^CGG
NciI 1989 CCGGG CC^[GC]GG
NlaIV 1496 GGTTCC GG[ACGT]^[ACGT]CC
PpuMI 1229 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1611 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2110 CAGCTG CAG^CTG
SapI 739 GCTCTTC GCTCTTC(1/4)
Sau96I 1230 GGACC G^G[ACGT]CC
SpeI 247 ACTAGT A^CTAGT
StyI 1782 CCTAGG C^C[AT][AT]GG
XmnI 736 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvI 2112 GCTGC GCAGC(8/12)
2115 GCTGC
BclI 80 TGATCA T^GATCA
119 TGATCA
BsaJI 1434 CCGAGG C^C[ACGT][ACGT]GG
1782 CCTAGG
BsgI 2116 CTGCAC GTGCAG(16/14)
1477 GTGCAG
BstYI 930 GGATCT [GA]^GATC[CT]
1318 AGATCT
Cac8I 1475 GCGTGC GC[ACGT]^[ACGT]GC
1797 GCTGGC
DraI 19 TTTAAA TTT^AAA
973 TTTAAA
FbaI 80 TGATCA T^GATCA
119 TGATCA
HaeIII 300 GGCC GG^CC
1503 GGCC
MflI 930 GGATCT [GA]^GATC[CT]
1318 AGATCT
PflMI 1943 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2174 CCATTGTGTGG
PleI 105 GACTC GAGTC(4/5)
2191 GACTC
SmlI 107 CTCAAG C^T[CT][GA]AG
430 CTTAAG
TseI 2112 GCTGC G^C[AT]GC
2115 GCTGC
Van91I 1943 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2174 CCATTGTGTGG
XbaI 645 TCTAGA T^CTAGA
1071 TCTAGA
XhoII 930 GGATCT [GA]^GATC[CT]
1318 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AluI ApoI BbsI
BfaI BpuAI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI DdeI
DpnII EarI EcoRII Fnu4HI FokI
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
Sau3AI ScrFI SfaNI SspI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I