##################################
# NO CUT ENZYMES ( 191 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr13I ClaI
CpoI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
ItaI KasI KpnI KspI MaeII
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI NarI
NciI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI TseI Tsp45I TspRI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 29 CGCG CG^CG
AciI 221 CCGC CCGC(-3/-1)
AfaI 242 GTAC GT^AC
Asp700I 94 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 49 CTAG C^TAG
BsaBI 268 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 109 ACCGGA [AT]^CCGG[AT]
Bsp1407I 241 TGTACA T^GTACA
BsrFI 40 GCCGGC [GA]^CCGG[CT]
BsrGI 241 TGTACA T^GTACA
BsrI 255 CCAGT ACTGG(1/-1)
BssKI 23 CCAGG ^CC[ACGT]GG
BstNI 23 CCAGG CC^[AT]GG
BstUI 29 CGCG CG^CG
CfoI 30 GCGC GCG^C
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
DdeI 329 CTCAG C^T[ACGT]AG
DpnII 283 GATC ^GATC
DraI 259 TTTAAA TTT^AAA
DrdI 64 GACAAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 212 GAAGAG CTCTTC(1/4)
EcoRII 23 CCAGG ^CC[AT]GG
EcoT14I 224 CCAAGG C^C[AT][AT]GG
HaeIII 228 GGCC GG^CC
HhaI 30 GCGC GCG^C
HinP1I 30 GCGC G^CGC
HinfI 99 GATTC G^A[ACGT]TC
Ksp632I 212 GAAGAG CTCTTC(1/4)
MaeI 49 CTAG C^TAG
MaeIII 217 GTAAC ^GT[ACGT]AC
MamI 268 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 283 GATC ^GATC
MseI 260 TTAA T^TAA
MvaI 23 CCAGG CC^[AT]GG
MvnI 29 CGCG CG^CG
MwoI 21 GCCCAGGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 40 GCCGGC GCC^GGC
NdeII 283 GATC ^GATC
NgoMIV 40 GCCGGC G^CCGGC
NheI 48 GCTAGC G^CTAGC
NspI 44 GCATGC [GA]CATG^[CT]
RsaI 242 GTAC GT^AC
Sau3AI 283 GATC ^GATC
ScrFI 23 CCAGG CC^[ACGT]GG
SfaNI 32 GCATC GCATC(5/9)
SphI 44 GCATGC GCATG^C
SspBI 241 TGTACA T^GTACA
StyI 224 CCAAGG C^C[AT][AT]GG
TfiI 99 GATTC G^A[AT]TC
Tru9I 260 TTAA T^TAA
XcmI 155 CCATCGAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 94 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AluI 51 AGCT AG^CT
303 AGCT
BsaJI 22 CCCAGG C^C[ACGT][ACGT]GG
224 CCAAGG
BstF5I 103 CATCC GGATG(2/0)
274 CATCC
FokI 103 CATCC GGATG(9/13)
274 CATCC
HapII 41 CCGG C^CGG
110 CCGG
HpaII 41 CCGG C^CGG
110 CCGG
MnlI 328 CCTC CCTC(7/6)
87 GAGG
MspI 41 CCGG C^CGG
110 CCGG
NlaIV 151 GGAACC GG[ACGT]^[ACGT]CC
168 GGAACC
TaqI 62 TCGA T^CGA
158 TCGA
TthHB8I 62 TCGA T^CGA
158 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
Cac8I MboII NlaIII Tsp509I