Restriction map for
pF1KB8929
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BamHI BbrPI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstYI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FseI HincII HindII
HindIII HpaI KpnI KspI MaeII
MaeIII MamI MfeI MflI MluI
MroI MseI MspA1I MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 555 GGCGCC G[GA]^CG[CT]C
AflIII 95 ACATGT A^C[GA][CT]GT
AvaII 11 GGACC G^G[AT]CC
AviII 653 TGCGCA TGC^GCA
BanI 555 GGCGCC G^G[CT][GA]CC
BbeI 555 GGCGCC GGCGC^C
BbvCI 624 GCTGAGG CCTCAGC(-5/-2)
BsaHI 555 GGCGCC G[GA]^CG[CT]C
BseRI 364 GAGGAG GAGGAG(10/8)
BsmBI 691 GAGACG CGTCTC(1/5)
BsmI 683 GCATTC GAATGC(1/-1)
BspLU11I 95 ACATGT A^CATGT
BspMI 461 ACCTGC ACCTGC(4/8)
BsrFI 620 ACCGGC [GA]^CCGG[CT]
BstAPI 699 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 344 CCAGGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 620 ACCGGC [GA]^CCGG[CT]
DpnII 166 GATC ^GATC
DraIII 437 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 6 CCACGG C^C[GA][CT]GG
FauI 630 GCGGG CCCGC(4/6)
FspI 653 TGCGCA TGC^GCA
HgaI 693 GACGC GACGC(5/10)
Hin1I 555 GGCGCC G[GA]^CG[CT]C
KasI 555 GGCGCC G^GCGCC
MboI 166 GATC ^GATC
MslI 645 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 555 GGCGCC GG^CGCC
NdeII 166 GATC ^GATC
NspI 95 ACATGT [GA]CATG^[CT]
PaeR7I 52 CTCGAG C^TCGAG
PleI 139 GAGTC GAGTC(4/5)
PpuMI 10 GGGACCT [GA]G^G[AT]CC[CT]
SapI 148 GAAGAGC GCTCTTC(1/4)
Sau3AI 166 GATC ^GATC
SfaNI 314 GCATC GCATC(5/9)
SfoI 555 GGCGCC GGC^GCC
TfiI 798 GATTC G^A[AT]TC
XhoI 52 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 499 CGCG CG^CG
745 CGCG
AlwNI 185 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
490 CAGCCTCTG
AocI 190 CCTGAGG CC^T[ACGT]AGG
802 CCTGAGG
ApaI 76 GGGCCC GGGCC^C
210 GGGCCC
BalI 39 TGGCCA TGG^CCA
518 TGGCCA
BcgI 165 CGATCTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
638 GCAACAACATCG
BfaI 88 CTAG C^TAG
769 CTAG
BpmI 688 CTGGAG CTGGAG(16/14)
709 CTGGAG
BsmAI 665 GAGAC GTCTC(1/5)
691 GAGAC
BstUI 499 CGCG CG^CG
745 CGCG
Bsu36I 190 CCTGAGG CC^T[ACGT]AGG
802 CCTGAGG
EarI 787 CTCTTC CTCTTC(1/4)
148 GAAGAG
Eco81I 190 CCTGAGG CC^T[ACGT]AGG
802 CCTGAGG
EcoNI 546 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
816 CCTCATCAAGG
HinfI 139 GAGTC G^A[ACGT]TC
798 GATTC
HphI 357 GGTGA GGTGA(8/7)
758 TCACC
Ksp632I 787 CTCTTC CTCTTC(1/4)
148 GAAGAG
MaeI 88 CTAG C^TAG
769 CTAG
MboII 148 GAAGA GAAGA(8/7)
788 TCTTC
MluNI 39 TGGCCA TGG^CCA
518 TGGCCA
MscI 39 TGGCCA TGG^CCA
518 TGGCCA
MvnI 499 CGCG CG^CG
745 CGCG
PstI 421 CTGCAG CTGCA^G
537 CTGCAG
SfiI 202 GGCCTCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
510 GGCCCCTTTGGCC
StuI 110 AGGCCT AGG^CCT
201 AGGCCT
TaqI 53 TCGA T^CGA
137 TCGA
TthHB8I 53 TCGA T^CGA
137 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaI BanII
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI SmaI
SmlI TseI TspRI XmaCI XmaI