Restriction map for
pF1KE0194
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstF5I BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MaeII MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 117 GTAC GT^AC
Asp700I 355 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 103 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 319 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 79 CTCCAG CTGGAG(16/14)
BsaBI 54 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 76 CTCCTC GAGGAG(10/8)
BsmFI 312 GTCCC GGGAC(10/14)
BsrI 227 CCAGT ACTGG(1/-1)
BstEII 100 GGTGACC G^GT[ACGT]ACC
BstPI 100 GGTGACC G^GT[ACGT]ACC
CfoI 513 GCGC GCG^C
DraII 170 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 170 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 100 GGTGACC G^GT[ACGT]ACC
EcoT14I 47 CCAAGG C^C[AT][AT]GG
HhaI 513 GCGC GCG^C
HinP1I 513 GCGC G^CGC
MaeIII 101 GTGAC ^GT[ACGT]AC
MamI 54 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 411 CATCACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 103 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 91 GACTC GAGTC(4/5)
RsaI 117 GTAC GT^AC
SexAI 425 ACCTGGT A^CC[AT]GGT
SmlI 271 CTTGAG C^T[CT][GA]AG
SspI 388 AATATT AAT^ATT
StyI 47 CCAAGG C^C[AT][AT]GG
Tsp45I 101 GTGAC ^GT[GC]AC
Tth111I 103 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 355 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AluI 18 AGCT AG^CT
448 AGCT
BbvI 16 GCAGC GCAGC(8/12)
446 GCAGC
BsaJI 47 CCAAGG C^C[ACGT][ACGT]GG
191 CCCTGG
BsiYI 36 CCTGGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
173 CCCTGCAGTGG
BslI 36 CCTGGAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
173 CCCTGCAGTGG
DpnII 60 GATC ^GATC
306 GATC
Fnu4HI 16 GCAGC GC^[ACGT]GC
446 GCAGC
HphI 100 GGTGA GGTGA(8/7)
384 GGTGA
ItaI 16 GCAGC GC^[ACGT]GC
446 GCAGC
MboI 60 GATC ^GATC
306 GATC
MseI 56 TTAA T^TAA
535 TTAA
NdeII 60 GATC ^GATC
306 GATC
NlaIV 372 GGCTCC GG[ACGT]^[ACGT]CC
432 GGAACC
PstI 175 CTGCAG CTGCA^G
205 CTGCAG
Sau3AI 60 GATC ^GATC
306 GATC
SfcI 175 CTGCAG C^T[GA][CT]AG
205 CTGCAG
Tru9I 56 TTAA T^TAA
535 TTAA
TseI 16 GCAGC G^C[AT]GC
446 GCAGC
TspRI 178 CAGTG CA[GC]TG[ACGT][ACGT]^
258 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI ApoI BssKI BstNI Cfr13I
DdeI EcoRI EcoRII HaeIII HinfI
MboII MnlI MvaI MwoI NlaIII
Sau96I ScrFI TfiI Tsp509I