Restriction map for
pF1KB6590
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI AlwNI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoT22I FbaI
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MroI
MspA1I MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PleI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 664 GACGTC GACGT^C
AccI 871 GTAGAC GT^[AC][GT]AC
AccII 1041 CGCG CG^CG
AcyI 664 GACGTC G[GA]^CG[CT]C
AgeI 877 ACCGGT A^CCGGT
Alw44I 1083 GTGCAC G^TGCAC
ApaLI 1083 GTGCAC G^TGCAC
AspHI 1083 GTGCAC G[AT]GC[AT]^C
AvaI 172 CCCGAG C^[CT]CG[GA]G
BcgI 1196 GCATTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1138 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 405 CTCCAG CTGGAG(16/14)
BsaBI 267 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 664 GACGTC G[GA]^CG[CT]C
BsaI 67 GGTCTC GGTCTC(1/5)
BsaWI 877 ACCGGT [AT]^CCGG[AT]
BsgI 348 GTGCAG GTGCAG(16/14)
BsiHKAI 1083 GTGCAC G[AT]GC[AT]^C
BsmBI 355 CGTCTC CGTCTC(1/5)
BsmI 113 GAATGC GAATGC(1/-1)
BsoBI 172 CCCGAG C^[CT]CG[GA]G
BsrFI 877 ACCGGT [GA]^CCGG[CT]
BstEII 615 GGTCACC G^GT[ACGT]ACC
BstPI 615 GGTCACC G^GT[ACGT]ACC
BstUI 1041 CGCG CG^CG
BstYI 98 GGATCT [GA]^GATC[CT]
CfoI 1040 GCGC GCG^C
Cfr10I 877 ACCGGT [GA]^CCGG[CT]
DraI 91 TTTAAA TTT^AAA
EcoO65I 615 GGTCACC G^GT[ACGT]ACC
EcoRI 699 GAATTC G^AATTC
EcoRV 1108 GATATC GAT^ATC
HapII 878 CCGG C^CGG
HhaI 1040 GCGC GCG^C
Hin1I 664 GACGTC G[GA]^CG[CT]C
HinP1I 1040 GCGC G^CGC
HpaII 878 CCGG C^CGG
MaeII 665 ACGT A^CGT
MamI 267 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 98 GGATCT [GA]^GATC[CT]
MseI 92 TTAA T^TAA
MspI 878 CCGG C^CGG
MvnI 1041 CGCG CG^CG
NcoI 1103 CCATGG C^CATGG
PinAI 877 ACCGGT A^CCGGT
PshAI 789 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 384 GCTCTTC GCTCTTC(1/4)
ScaI 254 AGTACT AGT^ACT
SfcI 1065 CTACAG C^T[GA][CT]AG
SfiI 1137 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 876 CACCGGTG C[GA]^CCGG[CT]G
SmlI 106 CTCAAG C^T[CT][GA]AG
StuI 929 AGGCCT AGG^CCT
Tru9I 92 TTAA T^TAA
XhoII 98 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 699 GAATTC [GA]^AATT[CT]
762 GAATTT
AfaI 59 GTAC GT^AC
255 GTAC
AlwI 228 GATCC GGATC(4/5)
98 GGATC
ApaI 981 GGGCCC GGGCC^C
1136 GGGCCC
ApoI 699 GAATTC [GA]^AATT[CT]
762 GAATTT
AvaII 169 GGTCC G^G[AT]CC
1126 GGACC
BalI 158 TGGCCA TGG^CCA
1091 TGGCCA
BanI 277 GGCACC G^G[CT][GA]CC
333 GGTGCC
BbsI 850 GAAGAC GAAGAC(2/6)
667 GTCTTC
BpuAI 850 GAAGAC GAAGAC(2/6)
667 GTCTTC
BseRI 219 CTCCTC GAGGAG(10/8)
339 CTCCTC
BsmAI 68 GTCTC GTCTC(1/5)
356 GTCTC
BstXI 236 CCAACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
252 CCAGTACTATGG
DdeI 472 CTCAG C^T[ACGT]AG
894 CTCAG
DsaI 491 CCGTGG C^C[GA][CT]GG
1103 CCATGG
MluNI 158 TGGCCA TGG^CCA
1091 TGGCCA
MscI 158 TGGCCA TGG^CCA
1091 TGGCCA
MslI 486 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CACGGAGATG
RsaI 59 GTAC GT^AC
255 GTAC
TaqI 389 TCGA T^CGA
629 TCGA
Tsp509I 700 AATT ^AATT
763 AATT
TthHB8I 389 TCGA T^CGA
629 TCGA
XcmI 1094 CCATCCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1130 CCACCTGGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PflMI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI TseI Tsp45I TspRI
Van91I