Restriction map for
pF1KB7855
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AlwI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BlnI BsaAI BsaBI BsaHI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
Hin1I HindIII HpaI KasI KpnI
MamI MfeI MluI MseI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1405 GTCGAC GT^[AC][GT]AC
AccII 126 CGCG CG^CG
AccIII 755 TCCGGA T^CCGGA
AcsI 853 GAATTC [GA]^AATT[CT]
Alw44I 730 GTGCAC G^TGCAC
ApaLI 730 GTGCAC G^TGCAC
ApoI 853 GAATTC [GA]^AATT[CT]
AviII 215 TGCGCA TGC^GCA
BalI 743 TGGCCA TGG^CCA
BglI 284 GCCGGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 919 GAGACC GGTCTC(1/5)
BseAI 755 TCCGGA T^CCGGA
BsgI 655 GTGCAG GTGCAG(16/14)
BsmI 462 GAATGC GAATGC(1/-1)
BspEI 755 TCCGGA T^CCGGA
BspHI 935 TCATGA T^CATGA
BsrFI 248 ACCGGC [GA]^CCGG[CT]
BssSI 262 CACGAG CACGAG(-5/-1)
BstEII 228 GGTCACC G^GT[ACGT]ACC
BstPI 228 GGTCACC G^GT[ACGT]ACC
BstUI 126 CGCG CG^CG
Cfr10I 248 ACCGGC [GA]^CCGG[CT]
CpoI 317 CGGACCG CG^G[AT]CCG
EaeI 743 TGGCCA [CT]^GGCC[GA]
EcoO65I 228 GGTCACC G^GT[ACGT]ACC
EcoRI 853 GAATTC G^AATTC
FspI 215 TGCGCA TGC^GCA
HaeII 119 GGCGCT [GA]GCGC^[CT]
HgaI 986 GACGC GACGC(5/10)
HincII 1405 GTCGAC GT[CT]^[GA]AC
HindII 1405 GTCGAC GT[CT]^[GA]AC
KspI 125 CCGCGG CCGC^GG
MluNI 743 TGGCCA TGG^CCA
MroI 755 TCCGGA T^CCGGA
MscI 743 TGGCCA TGG^CCA
MvnI 126 CGCG CG^CG
NdeI 914 CATATG CA^TATG
NspI 92 GCATGC [GA]CATG^[CT]
PflMI 339 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 877 GACGATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 879 CGATCG CGAT^CG
RcaI 935 TCATGA T^CATGA
RsrII 317 CGGACCG CG^G[AT]CCG
SacII 125 CCGCGG CCGC^GG
SalI 1405 GTCGAC G^TCGAC
SapI 436 GAAGAGC GCTCTTC(1/4)
SbfI 783 CCTGCAGG CCTGCA^GG
SmaI 1560 CCCGGG CCC^GGG
SmlI 580 CTCAAG C^T[CT][GA]AG
SphI 92 GCATGC GCATG^C
Sse8387I 783 CCTGCAGG CCTGCA^GG
TfiI 350 GAATC G^A[AT]TC
Tsp509I 854 AATT ^AATT
Van91I 339 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1607 CCATTGCGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1560 CCCGGG C^CCGGG
XmaI 1560 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 861 GTAC GT^AC
1485 GTAC
AocI 1252 CCTCAGG CC^T[ACGT]AGG
1489 CCTGAGG
ApaI 1517 GGGCCC GGGCC^C
1556 GGGCCC
AvaI 704 CCCGAG C^[CT]CG[GA]G
1560 CCCGGG
BbsI 1342 GAAGAC GAAGAC(2/6)
1594 GAAGAC
BcgI 456 CGACCTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
611 GCAGCTGCCTCG
BfaI 1237 CTAG C^TAG
1507 CTAG
BglII 584 AGATCT A^GATCT
632 AGATCT
BlpI 304 GCTGAGC GC^T[ACGT]AGC
1010 GCTCAGC
Bpu1102I 304 GCTGAGC GC^T[ACGT]AGC
1010 GCTCAGC
BpuAI 1342 GAAGAC GAAGAC(2/6)
1594 GAAGAC
BsaWI 755 TCCGGA [AT]^CCGG[AT]
887 ACCGGA
BsiEI 828 CGACCG CG[GA][CT]^CG
879 CGATCG
BsmAI 919 GAGAC GTCTC(1/5)
802 GTCTC
BsoBI 704 CCCGAG C^[CT]CG[GA]G
1560 CCCGGG
BspMI 863 ACCTGC ACCTGC(4/8)
1269 ACCTGC
BsrDI 990 CATTGC GCAATG(2/0)
1608 CATTGC
BstXI 42 CCAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCATGCTGATGG
BstYI 584 AGATCT [GA]^GATC[CT]
632 AGATCT
Bsu36I 1252 CCTCAGG CC^T[ACGT]AGG
1489 CCTGAGG
CelII 304 GCTGAGC GC^T[ACGT]AGC
1010 GCTCAGC
EarI 436 GAAGAG CTCTTC(1/4)
942 GAAGAG
Eco81I 1252 CCTCAGG CC^T[ACGT]AGG
1489 CCTGAGG
EcoNI 375 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1158 CCTGCCCCAGG
Ksp632I 436 GAAGAG CTCTTC(1/4)
942 GAAGAG
MaeI 1237 CTAG C^TAG
1507 CTAG
MaeII 903 ACGT A^CGT
1054 ACGT
MflI 584 AGATCT [GA]^GATC[CT]
632 AGATCT
PleI 945 GAGTC GAGTC(4/5)
1638 GAGTC
RsaI 861 GTAC GT^AC
1485 GTAC
StuI 44 AGGCCT AGG^CCT
1124 AGGCCT
TaqI 984 TCGA T^CGA
1406 TCGA
TthHB8I 984 TCGA T^CGA
1406 TCGA
XhoII 584 AGATCT [GA]^GATC[CT]
632 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PpuMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI